Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

Sunghwa Choe, Shozo Fujioka, Takahiro Noguchi, Suguru Takatsuto, Shigeo Yoshida, Kenneth A Feldmann

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

Plants unable to synthesize or perceive brassinosteroids (BRs) are dwarfs. Arabidopsis dwf4 was shown to be defective in a steroid 22α hydroxylase (CYP90B1) step that is the putative rate-limiting step in the BR biosynthetic pathway. To better understand the role of DWF4 in BR biosynthesis, transgenic Arabidopsis plants ectopically overexpressing DWF4 (AOD4) were generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes were characterized. The hypocotyl length of both light-and dark-grown AOD4 seedlings was increased dramatically as compared to wild type. At maturity, inflorescence height increased >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced. Analysis of endogenous BR levels in dwf4, Ws-2 and AOD4 revealed that dwf4 accumulated the precursors of the 22α-hydroxylation steps, whereas overexpression of DWF4 resulted in increased levels of downstream compounds relative to Ws-2, indicative of facilitated metabolic flow through the step. Both the levels of DWF4 transcripts and BR phenotypic effects were progressively increased in dwf4, wild-type and AOD4 plants, respectively. This suggests that it will be possible to control plant growth by engineering DWF4 transcription in plants.

Original languageEnglish (US)
Pages (from-to)573-582
Number of pages10
JournalPlant Journal
Volume26
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Brassinosteroids
brassinosteroids
Biosynthetic Pathways
Arabidopsis
seed yield
vegetative growth
biochemical pathways
Seeds
Growth
Steroid Hydroxylases
plant growth control
Caulimovirus
Hypocotyl
Inflorescence
Cauliflower mosaic virus
Genetically Modified Plants
hydroxylation
Hydroxylation
Seedlings
hypocotyls

Keywords

  • Brassinolide
  • Cell elongation
  • CYP90B1
  • Cytochrome P
  • DWARF4
  • Steroid hormones

ASJC Scopus subject areas

  • Plant Science

Cite this

Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. / Choe, Sunghwa; Fujioka, Shozo; Noguchi, Takahiro; Takatsuto, Suguru; Yoshida, Shigeo; Feldmann, Kenneth A.

In: Plant Journal, Vol. 26, No. 6, 2001, p. 573-582.

Research output: Contribution to journalArticle

Choe, Sunghwa ; Fujioka, Shozo ; Noguchi, Takahiro ; Takatsuto, Suguru ; Yoshida, Shigeo ; Feldmann, Kenneth A. / Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. In: Plant Journal. 2001 ; Vol. 26, No. 6. pp. 573-582.
@article{257994362202463c8d10f3d4de87a5f2,
title = "Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis",
abstract = "Plants unable to synthesize or perceive brassinosteroids (BRs) are dwarfs. Arabidopsis dwf4 was shown to be defective in a steroid 22α hydroxylase (CYP90B1) step that is the putative rate-limiting step in the BR biosynthetic pathway. To better understand the role of DWF4 in BR biosynthesis, transgenic Arabidopsis plants ectopically overexpressing DWF4 (AOD4) were generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes were characterized. The hypocotyl length of both light-and dark-grown AOD4 seedlings was increased dramatically as compared to wild type. At maturity, inflorescence height increased >35{\%} in AOD4 lines and >14{\%} in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59{\%} increase in the number of seeds produced. Analysis of endogenous BR levels in dwf4, Ws-2 and AOD4 revealed that dwf4 accumulated the precursors of the 22α-hydroxylation steps, whereas overexpression of DWF4 resulted in increased levels of downstream compounds relative to Ws-2, indicative of facilitated metabolic flow through the step. Both the levels of DWF4 transcripts and BR phenotypic effects were progressively increased in dwf4, wild-type and AOD4 plants, respectively. This suggests that it will be possible to control plant growth by engineering DWF4 transcription in plants.",
keywords = "Brassinolide, Cell elongation, CYP90B1, Cytochrome P, DWARF4, Steroid hormones",
author = "Sunghwa Choe and Shozo Fujioka and Takahiro Noguchi and Suguru Takatsuto and Shigeo Yoshida and Feldmann, {Kenneth A}",
year = "2001",
doi = "10.1046/j.1365-313X.2001.01055.x",
language = "English (US)",
volume = "26",
pages = "573--582",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

AU - Choe, Sunghwa

AU - Fujioka, Shozo

AU - Noguchi, Takahiro

AU - Takatsuto, Suguru

AU - Yoshida, Shigeo

AU - Feldmann, Kenneth A

PY - 2001

Y1 - 2001

N2 - Plants unable to synthesize or perceive brassinosteroids (BRs) are dwarfs. Arabidopsis dwf4 was shown to be defective in a steroid 22α hydroxylase (CYP90B1) step that is the putative rate-limiting step in the BR biosynthetic pathway. To better understand the role of DWF4 in BR biosynthesis, transgenic Arabidopsis plants ectopically overexpressing DWF4 (AOD4) were generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes were characterized. The hypocotyl length of both light-and dark-grown AOD4 seedlings was increased dramatically as compared to wild type. At maturity, inflorescence height increased >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced. Analysis of endogenous BR levels in dwf4, Ws-2 and AOD4 revealed that dwf4 accumulated the precursors of the 22α-hydroxylation steps, whereas overexpression of DWF4 resulted in increased levels of downstream compounds relative to Ws-2, indicative of facilitated metabolic flow through the step. Both the levels of DWF4 transcripts and BR phenotypic effects were progressively increased in dwf4, wild-type and AOD4 plants, respectively. This suggests that it will be possible to control plant growth by engineering DWF4 transcription in plants.

AB - Plants unable to synthesize or perceive brassinosteroids (BRs) are dwarfs. Arabidopsis dwf4 was shown to be defective in a steroid 22α hydroxylase (CYP90B1) step that is the putative rate-limiting step in the BR biosynthetic pathway. To better understand the role of DWF4 in BR biosynthesis, transgenic Arabidopsis plants ectopically overexpressing DWF4 (AOD4) were generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes were characterized. The hypocotyl length of both light-and dark-grown AOD4 seedlings was increased dramatically as compared to wild type. At maturity, inflorescence height increased >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced. Analysis of endogenous BR levels in dwf4, Ws-2 and AOD4 revealed that dwf4 accumulated the precursors of the 22α-hydroxylation steps, whereas overexpression of DWF4 resulted in increased levels of downstream compounds relative to Ws-2, indicative of facilitated metabolic flow through the step. Both the levels of DWF4 transcripts and BR phenotypic effects were progressively increased in dwf4, wild-type and AOD4 plants, respectively. This suggests that it will be possible to control plant growth by engineering DWF4 transcription in plants.

KW - Brassinolide

KW - Cell elongation

KW - CYP90B1

KW - Cytochrome P

KW - DWARF4

KW - Steroid hormones

UR - http://www.scopus.com/inward/record.url?scp=0034922518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034922518&partnerID=8YFLogxK

U2 - 10.1046/j.1365-313X.2001.01055.x

DO - 10.1046/j.1365-313X.2001.01055.x

M3 - Article

C2 - 11489171

AN - SCOPUS:0034922518

VL - 26

SP - 573

EP - 582

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 6

ER -