Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity

Michael L Heien, Paul E M Phillips, Garret D. Stuber, Andrew T. Seipel, R. Mark Wightman

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Abstract

The voltammetric responses of carbon-fiber microelectrodes with a 1.0 V and a 1.4 V anodic limit were compared in this study. Fast-scan cyclic voltammetry was used to characterize the response to dopamine and several other neurochemicals. An increase in the adsorption properties of the carbon fiber leads to an increase in sensitivity of 9 fold in vivo. However the temporal response of the sensor is slower with the more positive anodic limit. Increased electron transfer kinetics also causes a decrease in the relative sensitivity for dopamine vs. other neurochemicals, and a change in their cyclic voltammograms. Stimulated release in the caudate-putamen was pharmacologically characterized in vivo using Ro-04-1284 and pargyline, and was consistent with that expected for dopamine.

Original languageEnglish (US)
Pages (from-to)1413-1419
Number of pages7
JournalAnalyst
Volume128
Issue number12
DOIs
Publication statusPublished - Dec 2003
Externally publishedYes

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Heien, M. L., Phillips, P. E. M., Stuber, G. D., Seipel, A. T., & Wightman, R. M. (2003). Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity. Analyst, 128(12), 1413-1419. https://doi.org/10.1039/b307024g