Ovine somatomedin, multiplication-stimulating activity, and insulin promote skeletal muscle satellite cell proliferation in vitro

M. V. Dodson, Ronald E Allen, K. L. Hossner

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Abstract

Primary cultures of skeletal muscle satellite cells, the postnatal myogenic precursor cells, were induced to proliferate by exposure to physiological levels of somatomedins (Sms)/insulin-like growth factors (IGFs) and pharmacological levels of insulin. These polypeptides were included in medium containing horse serum as well as serum-free defined medium. Dexamethasone inclusion in the serum-containing medium facilitated the ovine Sm (oSm; P < 0.05) and the multiplication-stimulating activity/rat IGF-II (MSA/rIGF-II; P < 0.25) responses, but not the insulin proliferative response. In addition, data from defined medium studies indicate that satellite cells are more sensitive to both IGF moieties than insulin and that the proliferations induced by half-maximal concentrations of oSm and insulin were similar (P < 0.05), but both were different from the proliferation induced by MSA/rIGF-II (P < 0.05). In the presence of insulin concentrations that promote maximum proliferation, the addition of oSm did not produce an additive effect, whereas the addition of MSA/rIGF-II did produce a significant increase in satellite cell proliferation above that induced by insulin. MSA/rIGF-II may, therefore, be stimulating proliferation of satellite cells through a receptor system different from that serving insulin and oSm. Collectively, these data support the hypothesis that Sms/IGFs play an important role in the control of postnatal muscle growth by providing a link between these hormones and one of the significant target cells involved in this process.

Original languageEnglish (US)
Pages (from-to)2357-2363
Number of pages7
JournalEndocrinology
Volume117
Issue number6
StatePublished - 1985

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Skeletal Muscle Satellite Cells
Insulin-Like Growth Factor II
Somatomedins
Sheep
Cell Proliferation
Insulin
Serum-Free Culture Media
In Vitro Techniques
Serum
Dexamethasone
Horses
Hormones
Pharmacology
Muscles
Peptides

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ovine somatomedin, multiplication-stimulating activity, and insulin promote skeletal muscle satellite cell proliferation in vitro. / Dodson, M. V.; Allen, Ronald E; Hossner, K. L.

In: Endocrinology, Vol. 117, No. 6, 1985, p. 2357-2363.

Research output: Contribution to journalArticle

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