Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor

Patrick J. Collins, Michiel J J Kotterman, James A Field, Alan D W Dobson

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

The in vitro oxidation of the two polycyclic aromatic hydrocarbons anthracene and benzo[a]pyrene, which have ionization potentials of ≤7.45 eV, is catalyzed by laccases from Trametes versicolor. Crude laccase preparations were able to oxidize both anthracene and the potent carcinogen benzo[a]pyrene. Oxidation of benzo[a]pyrene was enhanced by the addition of the cooxidant 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), while an increased anthracene oxidizing ability was observed in the presence of the low-molecular-weight culture fluid ultrafiltrate. Two purified laccase isozymes from T. versicolor were found to have similar oxidative activities towards anthracene and benzo[a]pyrene. Oxidation of anthracene by the purified isozymes was enhanced in the presence of ABTS, while ABTS was essential for the oxidation of benzo[a]pyrene. In all cases anthraquinone was identified as the major end product of anthracene oxidation. These findings indicate that laccases may have a role in the oxidation of polycyclic aromatic hydrocarbons by white rut fungi.

Original languageEnglish (US)
Pages (from-to)4563-4567
Number of pages5
JournalApplied and Environmental Microbiology
Volume62
Issue number12
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Trametes
Coriolus versicolor
Laccase
laccase
Benzo(a)pyrene
pyrene
oxidation
Polycyclic Aromatic Hydrocarbons
polycyclic aromatic hydrocarbons
Isoenzymes
isozymes
PAH
Anthraquinones
anthraquinones
sulfonates
sulfonate
carcinogen
carcinogens
Carcinogens
ionization

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor. / Collins, Patrick J.; Kotterman, Michiel J J; Field, James A; Dobson, Alan D W.

In: Applied and Environmental Microbiology, Vol. 62, No. 12, 12.1996, p. 4563-4567.

Research output: Contribution to journalArticle

Collins, Patrick J. ; Kotterman, Michiel J J ; Field, James A ; Dobson, Alan D W. / Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor. In: Applied and Environmental Microbiology. 1996 ; Vol. 62, No. 12. pp. 4563-4567.
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