Oxidation of anthracene in water/solvent mixtures by the white-rot fungus, Bjerkandera sp. strain BOS55

J. A. Field, F. Boelsma, H. Baten, W. H. Rulkens

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Polycyclic aromatic hydrocarbons (PAH) are persistent priority pollutants of soil and sediments. The use of white-rot fungi has been proposed as a means of bioremediating PAH-polluted sites. However, higher PAH compounds of low bioavailability in polluted soil are biodegraded slowly. In order to enhance their bioavailability, PAH solubilization, can be increased in water/solvent mixtures. The oxidation of a model PAH compound, anthracene, in the presence of cosolvents by the white-rot fungus, Bjerkandera sp. strain BOS55 was investigated. Acetone and ethanol at 5% were toxic to this fungus when added at the time of inoculation. However, when solvents up to 20% (v/v) were added to 9-day-old cultures, ligninolytic activity as indicated by Poly R-478 dye decolorization and anthracene oxidation was evident for several days. Since 20% solvent was toxic to cells, the oxidation of anthracene can be attributed to extracellular peroxidases, which were shown to tolerate the solvent. Solvent additions of 11%-21% (v/v) acetone or ethanol increased the rate of anthracene bioconversion to anthraquinone in liquid medium by a factor of 2-3 compared to fungal cultures receiving 1%-3% solvent.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume44
Issue number1-2
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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