Oxidative inhibition of Ca2+-ATPase in the rabbit lens

D. Borchman, C. A. Paterson, N. A. Delamere

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 x 10-6 M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 x 10-4 M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide.

Original languageEnglish (US)
Pages (from-to)1633-1637
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume30
Issue number7
StatePublished - 1989

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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