Oxidative inhibition of Ca2+-ATPase in the rabbit lens

D. Borchman, C. A. Paterson, Nicholas A Delamere

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 x 10-6 M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 x 10-4 M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide.

Original languageEnglish (US)
Pages (from-to)1633-1637
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume30
Issue number7
StatePublished - 1989
Externally publishedYes

Fingerprint

Calcium-Transporting ATPases
Hydrogen Peroxide
Lenses
Rabbits
Catalase
Epithelium
Membranes
sodium-translocating ATPase

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Oxidative inhibition of Ca2+-ATPase in the rabbit lens. / Borchman, D.; Paterson, C. A.; Delamere, Nicholas A.

In: Investigative Ophthalmology and Visual Science, Vol. 30, No. 7, 1989, p. 1633-1637.

Research output: Contribution to journalArticle

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