Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo

Stephanie Nonas, Anna A. Birukova, Panfeng Fu, Jungjie Xing, Santipongse Chatchavalvanich, Valery N. Bochkov, Norbert Leitinger, Joe GN Garcia, Konstantin G. Birukov

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Abstract

Background: Mechanical ventilation at high tidal volume (HTV) may cause pulmonary capillary leakage and acute lung inflammation resulting in ventilator-induced lung injury. Besides blunting the Toll-like receptor-4-induced inflammatory cascade and lung dysfunction in a model of lipopolysaccharide-induced lung injury, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) exerts direct barrier-protective effects on pulmonary endothelial cells in vitro via activation of the small GTPases Rac and Cdc42. To test the hypothesis that OxPAPC may attenuate lung inflammation and barrier disruption caused by pathologic lung distension, we used a rodent model of ventilator-induced lung injury and an in vitro model of pulmonary endothelial cells exposed to pathologic mechanochemical stimulation. Methods: Rats received a single intravenous injection of OxPAPC (1.5 mg/kg) followed by mechanical ventilation at low tidal volume (LTV) (7 mL/kg) or HTV (20 mL/kg). Bronchoalveolar lavage was performed and lung tissue was stained for histological analysis. In vitro, the effects of OxPAPC on endothelial barrier dysfunction and GTPase activation were assessed in cells exposed to thrombin and pathologic (18%) cyclic stretch. Results: HTV induced profound increases in bronchoalveolar lavage and tissue neutrophils and in lavage protein. Intravenous OxPAPC markedly attenuated HTV-induced protein and inflammatory cell accumulation in bronchoalveolar lavage fluid and lung tissue. In vitro, high-magnitude stretch enhanced thrombin-induced endothelial paracellular gap formation associated with Rho activation. These effects were dramatically attenuated by OxPAPC and were associated with OxPAPC-induced activation of Rac. Conclusion: OxPAPC exhibits protective effects in these models of ventilator-induced lung injury.

Original languageEnglish (US)
Article numberR27
JournalCritical Care
Volume12
Issue number1
DOIs
StatePublished - Jan 24 2008
Externally publishedYes

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Phosphorylcholine
Mechanical Ventilators
Blood Vessels
Phospholipids
Tidal Volume
Inflammation
Ventilator-Induced Lung Injury
Lung
Bronchoalveolar Lavage
Artificial Respiration
Thrombin
Pneumonia
Endothelial Cells
Toll-Like Receptor 4
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Lung Injury
Intravenous Injections

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Medicine(all)

Cite this

Nonas, S., Birukova, A. A., Fu, P., Xing, J., Chatchavalvanich, S., Bochkov, V. N., ... Birukov, K. G. (2008). Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo. Critical Care, 12(1), [R27]. https://doi.org/10.1186/cc6805

Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo. / Nonas, Stephanie; Birukova, Anna A.; Fu, Panfeng; Xing, Jungjie; Chatchavalvanich, Santipongse; Bochkov, Valery N.; Leitinger, Norbert; Garcia, Joe GN; Birukov, Konstantin G.

In: Critical Care, Vol. 12, No. 1, R27, 24.01.2008.

Research output: Contribution to journalArticle

Nonas, S, Birukova, AA, Fu, P, Xing, J, Chatchavalvanich, S, Bochkov, VN, Leitinger, N, Garcia, JGN & Birukov, KG 2008, 'Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo', Critical Care, vol. 12, no. 1, R27. https://doi.org/10.1186/cc6805
Nonas S, Birukova AA, Fu P, Xing J, Chatchavalvanich S, Bochkov VN et al. Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo. Critical Care. 2008 Jan 24;12(1). R27. https://doi.org/10.1186/cc6805
Nonas, Stephanie ; Birukova, Anna A. ; Fu, Panfeng ; Xing, Jungjie ; Chatchavalvanich, Santipongse ; Bochkov, Valery N. ; Leitinger, Norbert ; Garcia, Joe GN ; Birukov, Konstantin G. / Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo. In: Critical Care. 2008 ; Vol. 12, No. 1.
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abstract = "Background: Mechanical ventilation at high tidal volume (HTV) may cause pulmonary capillary leakage and acute lung inflammation resulting in ventilator-induced lung injury. Besides blunting the Toll-like receptor-4-induced inflammatory cascade and lung dysfunction in a model of lipopolysaccharide-induced lung injury, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) exerts direct barrier-protective effects on pulmonary endothelial cells in vitro via activation of the small GTPases Rac and Cdc42. To test the hypothesis that OxPAPC may attenuate lung inflammation and barrier disruption caused by pathologic lung distension, we used a rodent model of ventilator-induced lung injury and an in vitro model of pulmonary endothelial cells exposed to pathologic mechanochemical stimulation. Methods: Rats received a single intravenous injection of OxPAPC (1.5 mg/kg) followed by mechanical ventilation at low tidal volume (LTV) (7 mL/kg) or HTV (20 mL/kg). Bronchoalveolar lavage was performed and lung tissue was stained for histological analysis. In vitro, the effects of OxPAPC on endothelial barrier dysfunction and GTPase activation were assessed in cells exposed to thrombin and pathologic (18{\%}) cyclic stretch. Results: HTV induced profound increases in bronchoalveolar lavage and tissue neutrophils and in lavage protein. Intravenous OxPAPC markedly attenuated HTV-induced protein and inflammatory cell accumulation in bronchoalveolar lavage fluid and lung tissue. In vitro, high-magnitude stretch enhanced thrombin-induced endothelial paracellular gap formation associated with Rho activation. These effects were dramatically attenuated by OxPAPC and were associated with OxPAPC-induced activation of Rac. Conclusion: OxPAPC exhibits protective effects in these models of ventilator-induced lung injury.",
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AU - Birukova, Anna A.

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AU - Xing, Jungjie

AU - Chatchavalvanich, Santipongse

AU - Bochkov, Valery N.

AU - Leitinger, Norbert

AU - Garcia, Joe GN

AU - Birukov, Konstantin G.

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N2 - Background: Mechanical ventilation at high tidal volume (HTV) may cause pulmonary capillary leakage and acute lung inflammation resulting in ventilator-induced lung injury. Besides blunting the Toll-like receptor-4-induced inflammatory cascade and lung dysfunction in a model of lipopolysaccharide-induced lung injury, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) exerts direct barrier-protective effects on pulmonary endothelial cells in vitro via activation of the small GTPases Rac and Cdc42. To test the hypothesis that OxPAPC may attenuate lung inflammation and barrier disruption caused by pathologic lung distension, we used a rodent model of ventilator-induced lung injury and an in vitro model of pulmonary endothelial cells exposed to pathologic mechanochemical stimulation. Methods: Rats received a single intravenous injection of OxPAPC (1.5 mg/kg) followed by mechanical ventilation at low tidal volume (LTV) (7 mL/kg) or HTV (20 mL/kg). Bronchoalveolar lavage was performed and lung tissue was stained for histological analysis. In vitro, the effects of OxPAPC on endothelial barrier dysfunction and GTPase activation were assessed in cells exposed to thrombin and pathologic (18%) cyclic stretch. Results: HTV induced profound increases in bronchoalveolar lavage and tissue neutrophils and in lavage protein. Intravenous OxPAPC markedly attenuated HTV-induced protein and inflammatory cell accumulation in bronchoalveolar lavage fluid and lung tissue. In vitro, high-magnitude stretch enhanced thrombin-induced endothelial paracellular gap formation associated with Rho activation. These effects were dramatically attenuated by OxPAPC and were associated with OxPAPC-induced activation of Rac. Conclusion: OxPAPC exhibits protective effects in these models of ventilator-induced lung injury.

AB - Background: Mechanical ventilation at high tidal volume (HTV) may cause pulmonary capillary leakage and acute lung inflammation resulting in ventilator-induced lung injury. Besides blunting the Toll-like receptor-4-induced inflammatory cascade and lung dysfunction in a model of lipopolysaccharide-induced lung injury, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) exerts direct barrier-protective effects on pulmonary endothelial cells in vitro via activation of the small GTPases Rac and Cdc42. To test the hypothesis that OxPAPC may attenuate lung inflammation and barrier disruption caused by pathologic lung distension, we used a rodent model of ventilator-induced lung injury and an in vitro model of pulmonary endothelial cells exposed to pathologic mechanochemical stimulation. Methods: Rats received a single intravenous injection of OxPAPC (1.5 mg/kg) followed by mechanical ventilation at low tidal volume (LTV) (7 mL/kg) or HTV (20 mL/kg). Bronchoalveolar lavage was performed and lung tissue was stained for histological analysis. In vitro, the effects of OxPAPC on endothelial barrier dysfunction and GTPase activation were assessed in cells exposed to thrombin and pathologic (18%) cyclic stretch. Results: HTV induced profound increases in bronchoalveolar lavage and tissue neutrophils and in lavage protein. Intravenous OxPAPC markedly attenuated HTV-induced protein and inflammatory cell accumulation in bronchoalveolar lavage fluid and lung tissue. In vitro, high-magnitude stretch enhanced thrombin-induced endothelial paracellular gap formation associated with Rho activation. These effects were dramatically attenuated by OxPAPC and were associated with OxPAPC-induced activation of Rac. Conclusion: OxPAPC exhibits protective effects in these models of ventilator-induced lung injury.

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