Abstract
Intraportal islet transplantation (IT) is not widely utilized as a treatment for type 1 diabetes. Oxygenation of the intraportally transplanted islet has not been studied extensively. We present a diffusion-reaction model that predicts the presence of an anoxic core and a larger partly functional core within intraportally transplanted islets. Four variables were studied: islet diameter, islet fractional viability, external oxygen partial pressure (P) (in surrounding portal blood), and presence or absence of a thrombus on the islet surface. Results indicate that an islet with average size and fractional viability exhibits an anoxic volume fraction (AVF) of 14% and a function loss of 72% at a low external P. Thrombus formation increased AVF to 30% and function loss to 92%, suggesting that the effect of thrombosis may be substantial. External P and islet diameter accounted for the greatest overall impact on AVF and loss of function. At our institutions, large human alloislets (>200 μm diameter) account for 20% of total islet number but 70% of total islet volume; since most of the total transplanted islet volume is accounted for by large islets, most of the intraportal islet cells are likely to be anoxic and not fully functional.
Original language | English (US) |
---|---|
Article number | 7625947 |
Journal | Journal of Diabetes Research |
Volume | 2016 |
DOIs | |
State | Published - 2016 |
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ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
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Oxygenation of the Intraportally Transplanted Pancreatic Islet. / Suszynski, Thomas M.; Avgoustiniatos, Efstathios S.; Papas, Klearchos K.
In: Journal of Diabetes Research, Vol. 2016, 7625947, 2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxygenation of the Intraportally Transplanted Pancreatic Islet
AU - Suszynski, Thomas M.
AU - Avgoustiniatos, Efstathios S.
AU - Papas, Klearchos K
PY - 2016
Y1 - 2016
N2 - Intraportal islet transplantation (IT) is not widely utilized as a treatment for type 1 diabetes. Oxygenation of the intraportally transplanted islet has not been studied extensively. We present a diffusion-reaction model that predicts the presence of an anoxic core and a larger partly functional core within intraportally transplanted islets. Four variables were studied: islet diameter, islet fractional viability, external oxygen partial pressure (P) (in surrounding portal blood), and presence or absence of a thrombus on the islet surface. Results indicate that an islet with average size and fractional viability exhibits an anoxic volume fraction (AVF) of 14% and a function loss of 72% at a low external P. Thrombus formation increased AVF to 30% and function loss to 92%, suggesting that the effect of thrombosis may be substantial. External P and islet diameter accounted for the greatest overall impact on AVF and loss of function. At our institutions, large human alloislets (>200 μm diameter) account for 20% of total islet number but 70% of total islet volume; since most of the total transplanted islet volume is accounted for by large islets, most of the intraportal islet cells are likely to be anoxic and not fully functional.
AB - Intraportal islet transplantation (IT) is not widely utilized as a treatment for type 1 diabetes. Oxygenation of the intraportally transplanted islet has not been studied extensively. We present a diffusion-reaction model that predicts the presence of an anoxic core and a larger partly functional core within intraportally transplanted islets. Four variables were studied: islet diameter, islet fractional viability, external oxygen partial pressure (P) (in surrounding portal blood), and presence or absence of a thrombus on the islet surface. Results indicate that an islet with average size and fractional viability exhibits an anoxic volume fraction (AVF) of 14% and a function loss of 72% at a low external P. Thrombus formation increased AVF to 30% and function loss to 92%, suggesting that the effect of thrombosis may be substantial. External P and islet diameter accounted for the greatest overall impact on AVF and loss of function. At our institutions, large human alloislets (>200 μm diameter) account for 20% of total islet number but 70% of total islet volume; since most of the total transplanted islet volume is accounted for by large islets, most of the intraportal islet cells are likely to be anoxic and not fully functional.
UR - http://www.scopus.com/inward/record.url?scp=84998865433&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84998865433&partnerID=8YFLogxK
U2 - 10.1155/2016/7625947
DO - 10.1155/2016/7625947
M3 - Article
C2 - 27872862
AN - SCOPUS:84998865433
VL - 2016
JO - Journal of Diabetes Research
JF - Journal of Diabetes Research
SN - 2314-6745
M1 - 7625947
ER -