P bodies promote stress granule assembly in Saccharomyces cerevisiae

J. Ross Buchan, Denise Muhlrad, Roy Parker

Research output: Contribution to journalArticle

316 Scopus citations

Abstract

Recent results indicate that nontranslating mRNAs in eukaryotic cells exist in distinct biochemical states that accumulate in P bodies and stress granules, although the nature of interactions between these particles is unknown. We demonstrate in Saccharomyces cerevisiae that RNA granules with similar protein composition and assembly mechanisms as mammalian stress granules form during glucose deprivation. Stress granule assembly is dependent on P-body formation, whereas P-body assembly is independent of stress granule formation. This suggests that stress granules primarily form from mRNPs in preexisting P bodies, which is also supported by the kinetics of P-body and stress granule formation both in yeast and mammalian cells. These observations argue that P bodies are important sites for decisions of mRNA fate and that stress granules, at least in yeast, primarily represent pools of mRNAs stalled in the process of reentry into translation from P bodies.

Original languageEnglish (US)
Pages (from-to)441-455
Number of pages15
JournalJournal of Cell Biology
Volume183
Issue number3
DOIs
StatePublished - Nov 3 2008

ASJC Scopus subject areas

  • Cell Biology

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