P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain

Margaret E Tome, Joseph M. Herndon, Charles P. Schaefer, Leigh M. Jacobs, Yifeng Zhang, Chelsea K. Jarvis, Thomas P Davis

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

P-glycoprotein (PgP), a drug efflux pump in blood-brain barrier endothelial cells, is a major clinical obstacle for effective central nervous system drug delivery. Identifying PgP regulatory pathways that can be exploited clinically is critical for improving central nervous system drug delivery. We previously found that PgP activity increases in rat brain microvessels concomitant with decreased central nervous system drug delivery in response to acute peripheral inflammatory pain. In the current study, we tested the hypothesis that PgP traffics to the luminal plasma membrane of the microvessel endothelial cells from intracellular stores during peripheral inflammatory pain. Using immunofluorescence microscopy, we detected PgP in endothelial cell nuclei and in the luminal plasma membrane in control animals. Following peripheral inflammatory pain, luminal PgP staining increased while staining in the nucleus decreased. Biochemical analysis of nuclear PgP content confirmed our visual observations. Peripheral inflammatory pain also increased endothelial cell luminal staining of polymerase 1 and transcript release factor/cavin1 and serum deprivation response protein/cavin2, two caveolar scaffold proteins, without changing caveolin1 or protein kinase C delta binding protein/cavin3 location. Our data (a) indicate that PgP traffics from stores in the nucleus to the endothelial cell luminal membrane in response to peripheral inflammatory pain; (b) provide an explanation for our previous observation that peripheral inflammatory pain inhibits central nervous system drug uptake; and (c) suggest a novel regulatory mechanism for PgP activity in rat brain.

Original languageEnglish (US)
Pages (from-to)1913-1928
Number of pages16
JournalJournal of Cerebral Blood Flow and Metabolism
Volume36
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

P-Glycoprotein
Endothelium
Cell Membrane
Pain
Central Nervous System Agents
Brain
Endothelial Cells
Staining and Labeling
Microvessels
Protein Kinase C-delta
Blood-Brain Barrier
Cell Nucleus
Fluorescence Microscopy
Carrier Proteins
Proteins
Serum

Keywords

  • Caveolin1
  • P-glycoprotein
  • polymerase 1 and transcript release factor/cavin1
  • protein kinase C delta binding protein/cavin3
  • serum deprivation response protein/cavin2
  • trafficking

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain. / Tome, Margaret E; Herndon, Joseph M.; Schaefer, Charles P.; Jacobs, Leigh M.; Zhang, Yifeng; Jarvis, Chelsea K.; Davis, Thomas P.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 36, No. 11, 01.11.2016, p. 1913-1928.

Research output: Contribution to journalArticle

Tome, Margaret E ; Herndon, Joseph M. ; Schaefer, Charles P. ; Jacobs, Leigh M. ; Zhang, Yifeng ; Jarvis, Chelsea K. ; Davis, Thomas P. / P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain. In: Journal of Cerebral Blood Flow and Metabolism. 2016 ; Vol. 36, No. 11. pp. 1913-1928.
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