PAH transport and fluid absorption by isolated perfused frog proximal renal tubules

J. M. Irish, William H Dantzler

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Para aminohippurate (PAH) transport and fluid absorption were studied in isolated, perfused frog (Rana catesbeiana) proximal renal tubules. With 2x10-5 M PAH in the bath, tubule fluid to bath (TF/B) concentration ratios averaged 3.0 and net secretion averaged 746x10-15 mol min-1 mm-1 in the proximal tubule. Net PAH secretion did not vary with perfusion rate. During PAH secretion, cell water PAH concentration exceeded that in the tubular fluid or bath, suggesting active transport into cells and subsequent diffusion into lumen. In accordance with this concept, luminal membrane permeability (3.8x10-5 cm s-1) calculated from perfusion studies was about 6 times greater than peritubular membrane permeability (0.66x10-5 cm s-1) determined from studies of PAH efflux from tubules with oil filled lumens. Net transepithelial PAH transport saturated at bath concentration of about 6x10-5 M. Addition of 20 mM urea to PAH bath concentration of 2x10-5 M reduced net PAH secretion by 32%. Fluid absorption in proximal tubules averaged 0.34 nl min-1 mm-1. Ouabain (10-4, 10-5 or 10-6 M) added to bath blocked fluid absorption. Fluid absorption was partially restored following removal of ouabain.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology
Pages1509-1516
Number of pages8
Volume230
Edition6
StatePublished - 1976

Fingerprint

Proximal Kidney Tubule
Baths
Anura
Ouabain
Permeability
Perfusion
Rana catesbeiana
Membranes
Active Biological Transport
Urea
Oils
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Irish, J. M., & Dantzler, W. H. (1976). PAH transport and fluid absorption by isolated perfused frog proximal renal tubules. In American Journal of Physiology (6 ed., Vol. 230, pp. 1509-1516)

PAH transport and fluid absorption by isolated perfused frog proximal renal tubules. / Irish, J. M.; Dantzler, William H.

American Journal of Physiology. Vol. 230 6. ed. 1976. p. 1509-1516.

Research output: Chapter in Book/Report/Conference proceedingChapter

Irish, JM & Dantzler, WH 1976, PAH transport and fluid absorption by isolated perfused frog proximal renal tubules. in American Journal of Physiology. 6 edn, vol. 230, pp. 1509-1516.
Irish JM, Dantzler WH. PAH transport and fluid absorption by isolated perfused frog proximal renal tubules. In American Journal of Physiology. 6 ed. Vol. 230. 1976. p. 1509-1516
Irish, J. M. ; Dantzler, William H. / PAH transport and fluid absorption by isolated perfused frog proximal renal tubules. American Journal of Physiology. Vol. 230 6. ed. 1976. pp. 1509-1516
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