PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development

D. D. Fenger, J. L. Carminati, D. L. Burney-Sigman, H. Kashevsky, J. L. Dines, Lisa K Elfring, T. L. Orr-Weaver

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Following completion of meiosis, DNA replication must be repressed until fertilization. In Drosophila, this replication block requires the products of the pan gu (png), plutonium (plu) and giant nuclei (gnu) genes. These genes also ensure that S phase oscillates with mitosis in the early division cycles of the embryo. We have identified the png gene and shown that it encodes a Ser/Thr protein kinase expressed only in ovaries and early embryos, and that the predicted extent of kinase activity in png mutants inversely correlates with the severity of the mutant phenotypes. The PLU and PNG proteins form a complex that has PNG-dependent kinase activity, and this activity is necessary for normal levels of mitotic cyclins. Our results reveal a novel protein kinase complex that controls S phase at the onset of development apparently by stabilizing mitotic cyclins.

Original languageEnglish (US)
Pages (from-to)4763-4774
Number of pages12
JournalDevelopment
Volume127
Issue number22
StatePublished - 2000
Externally publishedYes

Fingerprint

S Phase
Mitosis
Protein Kinases
Drosophila
Cyclins
Phosphotransferases
Embryonic Structures
Genes
Plutonium
Meiosis
DNA Replication
Fertilization
Ovary
Phenotype
Proteins

Keywords

  • Cell cycle
  • Cyclins A and B
  • DNA replication
  • Drosophila
  • Mitosis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Fenger, D. D., Carminati, J. L., Burney-Sigman, D. L., Kashevsky, H., Dines, J. L., Elfring, L. K., & Orr-Weaver, T. L. (2000). PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Development, 127(22), 4763-4774.

PAN GU : A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. / Fenger, D. D.; Carminati, J. L.; Burney-Sigman, D. L.; Kashevsky, H.; Dines, J. L.; Elfring, Lisa K; Orr-Weaver, T. L.

In: Development, Vol. 127, No. 22, 2000, p. 4763-4774.

Research output: Contribution to journalArticle

Fenger, DD, Carminati, JL, Burney-Sigman, DL, Kashevsky, H, Dines, JL, Elfring, LK & Orr-Weaver, TL 2000, 'PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development', Development, vol. 127, no. 22, pp. 4763-4774.
Fenger DD, Carminati JL, Burney-Sigman DL, Kashevsky H, Dines JL, Elfring LK et al. PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Development. 2000;127(22):4763-4774.
Fenger, D. D. ; Carminati, J. L. ; Burney-Sigman, D. L. ; Kashevsky, H. ; Dines, J. L. ; Elfring, Lisa K ; Orr-Weaver, T. L. / PAN GU : A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. In: Development. 2000 ; Vol. 127, No. 22. pp. 4763-4774.
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AU - Kashevsky, H.

AU - Dines, J. L.

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