PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development

D. D. Fenger, J. L. Carminati, D. L. Burney-Sigman, H. Kashevsky, J. L. Dines, Lisa K Elfring, T. L. Orr-Weaver

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46 Scopus citations

Abstract

Following completion of meiosis, DNA replication must be repressed until fertilization. In Drosophila, this replication block requires the products of the pan gu (png), plutonium (plu) and giant nuclei (gnu) genes. These genes also ensure that S phase oscillates with mitosis in the early division cycles of the embryo. We have identified the png gene and shown that it encodes a Ser/Thr protein kinase expressed only in ovaries and early embryos, and that the predicted extent of kinase activity in png mutants inversely correlates with the severity of the mutant phenotypes. The PLU and PNG proteins form a complex that has PNG-dependent kinase activity, and this activity is necessary for normal levels of mitotic cyclins. Our results reveal a novel protein kinase complex that controls S phase at the onset of development apparently by stabilizing mitotic cyclins.

Original languageEnglish (US)
Pages (from-to)4763-4774
Number of pages12
JournalDevelopment
Volume127
Issue number22
Publication statusPublished - 2000
Externally publishedYes

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Keywords

  • Cell cycle
  • Cyclins A and B
  • DNA replication
  • Drosophila
  • Mitosis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Fenger, D. D., Carminati, J. L., Burney-Sigman, D. L., Kashevsky, H., Dines, J. L., Elfring, L. K., & Orr-Weaver, T. L. (2000). PAN GU: A protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Development, 127(22), 4763-4774.