Pan-myeloid differentiation of human cord blood derived cd34hematopoietic stem and progenitor cells

Aditi Bapat, Nakia Keita, Shalini Sharma

Research output: Contribution to journalArticle

Abstract

Ex vivo differentiation of human hematopoietic stem cells is a widely used model for studying hematopoiesis. The protocol described here is for cytokine induced differentiation of CD34+ hematopoietic stem and progenitor cells to the four myeloid lineage cells. CD34+ cells are isolated from human umbilical cord blood and co-cultured with MS-5 stromal cells in the presence of cytokines. Immunophenotypic characterization of the stem and progenitor cells, and the differentiated myeloid lineage cells are described. Using this protocol, CD34+ cells may be incubated with small molecules or transduced with lentiviruses to express myeloid disease mutations to investigate their impact on myeloid differentiation.

Original languageEnglish (US)
Article numbere59836
JournalJournal of Visualized Experiments
Volume2019
Issue number150
DOIs
StatePublished - Aug 2019

Keywords

  • CD34 cells
  • Common myeloid progenitor
  • Developmental Biology
  • Erythrocyte
  • Flow cytometry
  • Granulocyte
  • Granulocyte monocyte progenitor
  • Hematopoietic stem and progenitor cells
  • Issue 150
  • Megakaryocyte
  • Megakaryocyte erythroid progenitor
  • Monocyte
  • Myeloid differentiation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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