Panx1 regulates cellular properties of keratinocytes and dermal fibroblasts in skin development and wound healing

Silvia Penuela, John J. Kelly, Jared M. Churko, Kevin J. Barr, Amy C. Berger, Dale W. Laird

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Pannexin1 (Panx1), a channel-forming glycoprotein is expressed in neonatal but not in aged mouse skin. Histological staining of Panx1 knockout (KO) mouse skin revealed a reduction in epidermal and dermal thickness and an increase in hypodermal adipose tissue. Following dorsal skin punch biopsies, mutant mice exhibited a significant delay in wound healing. Scratch wound and proliferation assays revealed that cultured keratinocytes from KO mice were more migratory, whereas dermal fibroblasts were more proliferative compared with controls. In addition, collagen gels populated with fibroblasts from KO mice exhibited significantly reduced contraction, comparable to WT fibroblasts treated with the Panx1 blocker, probenecid. KO fibroblasts did not increase α-smooth muscle actin expression in response to TGF-β, as is the case for differentiating WT myofibroblasts during wound contraction. We conclude that Panx1 controls cellular properties of keratinocytes and dermal fibroblasts during early stages of skin development and modulates wound repair upon injury.

Original languageEnglish (US)
Pages (from-to)2026-2035
Number of pages10
JournalJournal of Investigative Dermatology
Volume134
Issue number7
DOIs
StatePublished - Jul 2014
Externally publishedYes

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Fibroblasts
Keratinocytes
Wound Healing
Skin
Knockout Mice
Wounds and Injuries
Probenecid
Biopsy
Muscle
Actins
Assays
Myofibroblasts
Glycoproteins
Repair
Collagen
Gels
Tissue
Smooth Muscle
Adipose Tissue
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Panx1 regulates cellular properties of keratinocytes and dermal fibroblasts in skin development and wound healing. / Penuela, Silvia; Kelly, John J.; Churko, Jared M.; Barr, Kevin J.; Berger, Amy C.; Laird, Dale W.

In: Journal of Investigative Dermatology, Vol. 134, No. 7, 07.2014, p. 2026-2035.

Research output: Contribution to journalArticle

Penuela, Silvia ; Kelly, John J. ; Churko, Jared M. ; Barr, Kevin J. ; Berger, Amy C. ; Laird, Dale W. / Panx1 regulates cellular properties of keratinocytes and dermal fibroblasts in skin development and wound healing. In: Journal of Investigative Dermatology. 2014 ; Vol. 134, No. 7. pp. 2026-2035.
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