Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus

Christopher F. Fronczek, Tu San Park, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were Iysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10% poultry packaging liquid extracted in cellulose paper was 103 CFU/mL, which was significantly lower than that of 106 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1114-1116
Number of pages3
Volume2
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Nucleic acids
Microfluidics
Nucleic Acids
Nitrocellulose
DNA
Collodion
Cellulose
Poultry
Salmonella
Packaging
Buffers
Plasmids
Liquids

Keywords

  • Cellulose
  • Direct detection
  • Influenza A
  • Nitrocellulose
  • Salmonella

ASJC Scopus subject areas

  • Bioengineering

Cite this

Fronczek, C. F., Park, T. S., & Yoon, J-Y. (2013). Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 1114-1116). Chemical and Biological Microsystems Society.

Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. / Fronczek, Christopher F.; Park, Tu San; Yoon, Jeong-Yeol.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 1114-1116.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fronczek, CF, Park, TS & Yoon, J-Y 2013, Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 2, Chemical and Biological Microsystems Society, pp. 1114-1116, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Fronczek CF, Park TS, Yoon J-Y. Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2. Chemical and Biological Microsystems Society. 2013. p. 1114-1116
Fronczek, Christopher F. ; Park, Tu San ; Yoon, Jeong-Yeol. / Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. pp. 1114-1116
@inproceedings{304d496182324e4fb0d1d6d2e695df4b,
title = "Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus",
abstract = "We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were Iysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10{\%} poultry packaging liquid extracted in cellulose paper was 103 CFU/mL, which was significantly lower than that of 106 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.",
keywords = "Cellulose, Direct detection, Influenza A, Nitrocellulose, Salmonella",
author = "Fronczek, {Christopher F.} and Park, {Tu San} and Jeong-Yeol Yoon",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
volume = "2",
pages = "1114--1116",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus

AU - Fronczek, Christopher F.

AU - Park, Tu San

AU - Yoon, Jeong-Yeol

PY - 2013

Y1 - 2013

N2 - We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were Iysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10% poultry packaging liquid extracted in cellulose paper was 103 CFU/mL, which was significantly lower than that of 106 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.

AB - We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were Iysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10% poultry packaging liquid extracted in cellulose paper was 103 CFU/mL, which was significantly lower than that of 106 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.

KW - Cellulose

KW - Direct detection

KW - Influenza A

KW - Nitrocellulose

KW - Salmonella

UR - http://www.scopus.com/inward/record.url?scp=84907334649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907334649&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781632666246

VL - 2

SP - 1114

EP - 1116

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -