Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus

Christopher F. Fronczek, Tu San Park, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were Iysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10% poultry packaging liquid extracted in cellulose paper was 103 CFU/mL, which was significantly lower than that of 106 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1114-1116
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Cellulose
  • Direct detection
  • Influenza A
  • Nitrocellulose
  • Salmonella

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Fronczek, C. F., Park, T. S., & Yoon, J. Y. (2013). Paper microfluidic extraction of bacterial and viral nucleic acid from field and clinical samples towards a direct microtas apparatus. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1114-1116). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.