Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community.

Original languageEnglish (US)
Pages (from-to)10221-10232
Number of pages12
JournalOptics Express
Volume22
Issue number9
DOIs
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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