Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii

Christopher D. Dupont, David A. Christian, Elizabeth M. Selleck, Marion Pepper, Michael Leney-Greene, Gretchen Harms Pritchard, Anita A Koshy, Sagie Wagage, Morgan A. Reuter, L. David Sibley, Michael R. Betts, Christopher A. Hunter

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

During infection with the intracellular parasite Toxoplasma gondii, the presentation of parasite-derived antigens to CD4+ and CD8+ T cells is essential for long-term resistance to this pathogen. Fundamental questions remain regarding the roles of phagocytosis and active invasion in the events that lead to the processing and presentation of parasite antigens. To understand the most proximal events in this process, an attenuated non-replicating strain of T. gondii (the cpsII strain) was combined with a cytometry-based approach to distinguish active invasion from phagocytic uptake. In vivo studies revealed that T. gondii disproportionately infected dendritic cells and macrophages, and that infected dendritic cells and macrophages displayed an activated phenotype characterized by enhanced levels of CD86 compared to cells that had phagocytosed the parasite, thus suggesting a role for these cells in priming naïve T cells. Indeed, dendritic cells were required for optimal CD4+ and CD8+ T cell responses, and the phagocytosis of heat-killed or invasion-blocked parasites was not sufficient to induce T cell responses. Rather, the selective transfer of cpsII-infected dendritic cells or macrophages (but not those that had phagocytosed the parasite) to naïve mice potently induced CD4+ and CD8+ T cell responses, and conferred protection against challenge with virulent T. gondii. Collectively, these results point toward a critical role for actively infected host cells in initiating T. gondii-specific CD4+ and CD8+ T cell responses.

Original languageEnglish (US)
Article numbere1004047
JournalPLoS Pathogens
Volume10
Issue number4
DOIs
StatePublished - 2014

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Toxoplasma
Parasites
T-Lymphocytes
Dendritic Cells
Cytophagocytosis
Macrophages
Phagocytosis
CD8 Antigens
CD4 Antigens
Antigen Presentation
Hot Temperature
Phenotype
Infection

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Dupont, C. D., Christian, D. A., Selleck, E. M., Pepper, M., Leney-Greene, M., Harms Pritchard, G., ... Hunter, C. A. (2014). Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii. PLoS Pathogens, 10(4), [e1004047]. https://doi.org/10.1371/journal.ppat.1004047

Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii. / Dupont, Christopher D.; Christian, David A.; Selleck, Elizabeth M.; Pepper, Marion; Leney-Greene, Michael; Harms Pritchard, Gretchen; Koshy, Anita A; Wagage, Sagie; Reuter, Morgan A.; Sibley, L. David; Betts, Michael R.; Hunter, Christopher A.

In: PLoS Pathogens, Vol. 10, No. 4, e1004047, 2014.

Research output: Contribution to journalArticle

Dupont, CD, Christian, DA, Selleck, EM, Pepper, M, Leney-Greene, M, Harms Pritchard, G, Koshy, AA, Wagage, S, Reuter, MA, Sibley, LD, Betts, MR & Hunter, CA 2014, 'Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii', PLoS Pathogens, vol. 10, no. 4, e1004047. https://doi.org/10.1371/journal.ppat.1004047
Dupont, Christopher D. ; Christian, David A. ; Selleck, Elizabeth M. ; Pepper, Marion ; Leney-Greene, Michael ; Harms Pritchard, Gretchen ; Koshy, Anita A ; Wagage, Sagie ; Reuter, Morgan A. ; Sibley, L. David ; Betts, Michael R. ; Hunter, Christopher A. / Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii. In: PLoS Pathogens. 2014 ; Vol. 10, No. 4.
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