Parasitism of diapausing pink bollworm Pectinophora gossypiella (Lepidoptera

Gelechiidae) larvae by entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae)

Dawn H Gouge, Linda L. Lee, Thomas J. Henneberry

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Diapausing larvae of Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), were exposed to entomopathogenic nematodes (Nematoda: Steinernematidae; Heterorhabditidae) at a dosage of 100 or 200 infective juveniles (IJ) per larva. No significant differences between infection of Steinernema riobrave (strain 355) Cabanillas, Poinar and Raulston, and Heterorhabditis bacteriophora (strain Cruiser) (Poinar) were observed after either 6-d or 9-d of incubation. No significant difference in infection levels occurred due to increasing dose. Greatest insect mortality occurred due to parasitism by S. riobrave (355). In a second assay, diapausing larvae of P. gossypiella were exposed to S. riobrave (355), Steinernema carpocapsae (strain Kapow) (Weiser), H. bacteriophora (strain Lawn Patrol), or H. bacteriophora (Cruiser) at dosages of 50, 100,200, or 400 IJs per larva. S. carpocapsae and H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with H. bacteriophora (Lawn Patrol) and S. riobrave. A significant difference in infection levels occurred due to nematode dose. In a third assay cotton bolls infested with diapausing P, gossypiella were exposed to S, riobrave (355), or H. bacteriophora (Cruiser). Boxes were prepared with bolls buried 5 cm below the soil surface or bolls laid on the soil surface. Boxes also were incubated at constant temperature or exposed to ambient seasonal temperatures and light. H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with S. riobrave (355). Significant differences in infection levels occurred due to the location of the boll placement and the incubation temperature.

Original languageEnglish (US)
Pages (from-to)531-537
Number of pages7
JournalCrop Protection
Volume18
Issue number8
DOIs
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Heterorhabditidae
Pectinophora gossypiella
Steinernematidae
Heterorhabditis bacteriophora
Gelechiidae
entomopathogenic nematodes
Steinernema riobravis
parasitism
Lepidoptera
Nematoda
bolls
larvae
Steinernema carpocapsae
dosage
infection
temperature
nematode larvae
assays
soil
cotton

Keywords

  • Entomopathogenic nematodes
  • Heterorhabditis
  • Infectivity
  • Insect diapause
  • Pectinophora gossypiella
  • Steinernema
  • Temperature

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{5561231508c04a97b30c7f320c3cd06d,
title = "Parasitism of diapausing pink bollworm Pectinophora gossypiella (Lepidoptera: Gelechiidae) larvae by entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae)",
abstract = "Diapausing larvae of Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), were exposed to entomopathogenic nematodes (Nematoda: Steinernematidae; Heterorhabditidae) at a dosage of 100 or 200 infective juveniles (IJ) per larva. No significant differences between infection of Steinernema riobrave (strain 355) Cabanillas, Poinar and Raulston, and Heterorhabditis bacteriophora (strain Cruiser) (Poinar) were observed after either 6-d or 9-d of incubation. No significant difference in infection levels occurred due to increasing dose. Greatest insect mortality occurred due to parasitism by S. riobrave (355). In a second assay, diapausing larvae of P. gossypiella were exposed to S. riobrave (355), Steinernema carpocapsae (strain Kapow) (Weiser), H. bacteriophora (strain Lawn Patrol), or H. bacteriophora (Cruiser) at dosages of 50, 100,200, or 400 IJs per larva. S. carpocapsae and H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with H. bacteriophora (Lawn Patrol) and S. riobrave. A significant difference in infection levels occurred due to nematode dose. In a third assay cotton bolls infested with diapausing P, gossypiella were exposed to S, riobrave (355), or H. bacteriophora (Cruiser). Boxes were prepared with bolls buried 5 cm below the soil surface or bolls laid on the soil surface. Boxes also were incubated at constant temperature or exposed to ambient seasonal temperatures and light. H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with S. riobrave (355). Significant differences in infection levels occurred due to the location of the boll placement and the incubation temperature.",
keywords = "Entomopathogenic nematodes, Heterorhabditis, Infectivity, Insect diapause, Pectinophora gossypiella, Steinernema, Temperature",
author = "Gouge, {Dawn H} and Lee, {Linda L.} and Henneberry, {Thomas J.}",
year = "1999",
month = "9",
doi = "10.1016/S0261-2194(99)00057-5",
language = "English (US)",
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journal = "Crop Protection",
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TY - JOUR

T1 - Parasitism of diapausing pink bollworm Pectinophora gossypiella (Lepidoptera

T2 - Gelechiidae) larvae by entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae)

AU - Gouge, Dawn H

AU - Lee, Linda L.

AU - Henneberry, Thomas J.

PY - 1999/9

Y1 - 1999/9

N2 - Diapausing larvae of Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), were exposed to entomopathogenic nematodes (Nematoda: Steinernematidae; Heterorhabditidae) at a dosage of 100 or 200 infective juveniles (IJ) per larva. No significant differences between infection of Steinernema riobrave (strain 355) Cabanillas, Poinar and Raulston, and Heterorhabditis bacteriophora (strain Cruiser) (Poinar) were observed after either 6-d or 9-d of incubation. No significant difference in infection levels occurred due to increasing dose. Greatest insect mortality occurred due to parasitism by S. riobrave (355). In a second assay, diapausing larvae of P. gossypiella were exposed to S. riobrave (355), Steinernema carpocapsae (strain Kapow) (Weiser), H. bacteriophora (strain Lawn Patrol), or H. bacteriophora (Cruiser) at dosages of 50, 100,200, or 400 IJs per larva. S. carpocapsae and H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with H. bacteriophora (Lawn Patrol) and S. riobrave. A significant difference in infection levels occurred due to nematode dose. In a third assay cotton bolls infested with diapausing P, gossypiella were exposed to S, riobrave (355), or H. bacteriophora (Cruiser). Boxes were prepared with bolls buried 5 cm below the soil surface or bolls laid on the soil surface. Boxes also were incubated at constant temperature or exposed to ambient seasonal temperatures and light. H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with S. riobrave (355). Significant differences in infection levels occurred due to the location of the boll placement and the incubation temperature.

AB - Diapausing larvae of Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), were exposed to entomopathogenic nematodes (Nematoda: Steinernematidae; Heterorhabditidae) at a dosage of 100 or 200 infective juveniles (IJ) per larva. No significant differences between infection of Steinernema riobrave (strain 355) Cabanillas, Poinar and Raulston, and Heterorhabditis bacteriophora (strain Cruiser) (Poinar) were observed after either 6-d or 9-d of incubation. No significant difference in infection levels occurred due to increasing dose. Greatest insect mortality occurred due to parasitism by S. riobrave (355). In a second assay, diapausing larvae of P. gossypiella were exposed to S. riobrave (355), Steinernema carpocapsae (strain Kapow) (Weiser), H. bacteriophora (strain Lawn Patrol), or H. bacteriophora (Cruiser) at dosages of 50, 100,200, or 400 IJs per larva. S. carpocapsae and H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with H. bacteriophora (Lawn Patrol) and S. riobrave. A significant difference in infection levels occurred due to nematode dose. In a third assay cotton bolls infested with diapausing P, gossypiella were exposed to S, riobrave (355), or H. bacteriophora (Cruiser). Boxes were prepared with bolls buried 5 cm below the soil surface or bolls laid on the soil surface. Boxes also were incubated at constant temperature or exposed to ambient seasonal temperatures and light. H. bacteriophora (Cruiser) infected larvae at significantly higher rates compared with S. riobrave (355). Significant differences in infection levels occurred due to the location of the boll placement and the incubation temperature.

KW - Entomopathogenic nematodes

KW - Heterorhabditis

KW - Infectivity

KW - Insect diapause

KW - Pectinophora gossypiella

KW - Steinernema

KW - Temperature

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DO - 10.1016/S0261-2194(99)00057-5

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JF - Crop Protection

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