Partially unfolded species populated during equilibrium denaturation of the β-sheet protein Y74W apo-pseudoazurin

Susan Jones, John S. Reader, Maria Healy, Andrew P. Capaldi, Alison E. Ashcroft, Arnout P. Kalverda, D. Alastair Smith, Sheena E. Radford

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Apo-pseudoazurin is a single domain cupredoxin. We have engineered a mutant in which a unique tryptophan replaces the tyrosine residue found in the tyrosine comer of this Greek key protein, a region that has been proposed to have an important role in folding. Equilibrium denaturation of Y74W apo- pseudoazurin demonstrated multistate unfolding in urea (pH 7.0, 0.5 M Na2SO4 at 15 °C), in which one or more partially folded species are populated in 4.3 M urea. Using a variety of biophysical techniques, we show that these species, on average, have lost a substantial portion of the native secondary structure, lack fixed tertiary packing involving tryptophan and tyrosine residues, are less compact than the native state as determined by fluorescence lifetimes and time-resolved anisotropy, but retain significant residual structure involving the trytophan residue. Peptides ranging in length from 11 to 30 residues encompassing this region, however, did not contain detectable nonrandom structure, suggesting that long-range interactions are important for stabilizing the equilibrium partially unfolded species in the intact protein. On the basis of these results, we suggest that the equilibrium denaturation of Y74W apo-pseudoazurin generates one or more partially unfolded species that are globally collapsed and retain elements of the native structure involving the newly introduced tryptophan residue. We speculate on the role of such intermediates in the generation of the complex Greek key fold.

Original languageEnglish (US)
Pages (from-to)5672-5682
Number of pages11
JournalBiochemistry
Volume39
Issue number19
DOIs
StatePublished - May 16 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Jones, S., Reader, J. S., Healy, M., Capaldi, A. P., Ashcroft, A. E., Kalverda, A. P., Smith, D. A., & Radford, S. E. (2000). Partially unfolded species populated during equilibrium denaturation of the β-sheet protein Y74W apo-pseudoazurin. Biochemistry, 39(19), 5672-5682. https://doi.org/10.1021/bi9923959