Passion fruit peel extract attenuates bleomycin-induced pulmonary fibrosis in mice

Shanmuga Reddy Chilakapati, Mamatha Serasanambati, Pavan Kumar Manikonda, Damodar Reddy Chilakapati, Ronald Ross Watson

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Idiopathic pulmonary fibrosis is a progressive fatal lung disease characterized by excessive collagen deposition, with no effective treatments. We investigated the efficacy of natural products with high anti-inflammatory activity, such as passion fruit peel extract (PFPE), in a mouse model of bleomycin-induced pulmonary fibrosis (PF). C57BL/6J mice were subjected to a single intratracheal instillation of bleomycin to induce PF. Daily PFPE treatment significantly reduced loss of body mass and mortality rate in mice compared with those treated with bleomycin. While bleomycin-induced PF resulted in elevated total numbers of inflammatory cells, macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid on both days 7 and 21, PFPE administration significantly attenuated these phenomena compared with bleomycin group. On day 7, the decreased superoxide dismutase and myeloperoxidase activities observed in the bleomycin group were significantly restored with PFPE treatment. On day 21, enhanced hydroxyproline deposition in the bleomycin group was also suppressed by PFPE administration. PFPE treatment significantly attenuated extensive inflammatory cell infiltration and accumulation of collagen in lung tissue sections of bleomycin-induced mice on days 7 and 21, respectively. Our results indicate that administration of PFPE decreased bleomycin-induced PF because of anti-inflammatory and antioxidant activities.

Original languageEnglish (US)
Pages (from-to)631-639
Number of pages9
JournalCanadian Journal of Physiology and Pharmacology
Volume92
Issue number8
DOIs
StatePublished - Aug 2014

Keywords

  • Bleomycin
  • Bronchoalveolar lavage fluid
  • Idiopathic pulmonary fibrosis
  • Myeloperoxidase
  • Passion fruit
  • Superoxide dismutase

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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