Abstract
A previous study on alfalfa determined that the number of plastids/generative cell does not necessarily correlate with male plastid transmission strength in a given genotype. The objectives of the present study were to learn (1) whether plastid nucleoid number/generative cell is comparable to the number of plastids/generative cell, and (2) whether plastid nucleoid number/generative cell correlates with known male plastid transmission behavior in three alfalfa genotypes. Our results, which were based upon 150 generative cells examined by DAPI/epifluorescence microscopy, indicate that the mean plastid nucleoid number/generative cell is much less than the mean number of plastids/generative cell in genotype 7W (60 nucleoids/264 plastids) and genotype 301 (54 nucleoids/165 plastids). In genotype MS-5, mean plastid nucleoid number/generative cell (45) is similar to the mean number of plastids/generative cell (65). The significantly fewer plastid nucleoids/generative cell in MS-5, compared to that of 7W and 301, correlates positively with the relatively poor male plastid transmission strength of this genotype. However, the difference between the mean number of plastid nucleoids/generative cell in 7W and 301 is not significant, yet 301 is a much stronger transmitter of male plastids than is 7W.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 399-401 |
| Number of pages | 3 |
| Journal | Current Genetics |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1991 |
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Keywords
- Cytoplasmic inheritance
- DAPI fluorochrome
- Medicago sativa
- Plastid DNA
ASJC Scopus subject areas
- Genetics
Cite this
Paternal plastid inheritance in alfalfa : plastid nucleoid number within generative cells correlates poorly with plastid number and male plastid transmission trength. / Shi, Liang; Zhu, Tong; Mogensen, H. Lloyd; Smith, Steven E.
In: Current Genetics, Vol. 19, No. 5, 05.1991, p. 399-401.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Paternal plastid inheritance in alfalfa
T2 - plastid nucleoid number within generative cells correlates poorly with plastid number and male plastid transmission trength
AU - Shi, Liang
AU - Zhu, Tong
AU - Mogensen, H. Lloyd
AU - Smith, Steven E
PY - 1991/5
Y1 - 1991/5
N2 - A previous study on alfalfa determined that the number of plastids/generative cell does not necessarily correlate with male plastid transmission strength in a given genotype. The objectives of the present study were to learn (1) whether plastid nucleoid number/generative cell is comparable to the number of plastids/generative cell, and (2) whether plastid nucleoid number/generative cell correlates with known male plastid transmission behavior in three alfalfa genotypes. Our results, which were based upon 150 generative cells examined by DAPI/epifluorescence microscopy, indicate that the mean plastid nucleoid number/generative cell is much less than the mean number of plastids/generative cell in genotype 7W (60 nucleoids/264 plastids) and genotype 301 (54 nucleoids/165 plastids). In genotype MS-5, mean plastid nucleoid number/generative cell (45) is similar to the mean number of plastids/generative cell (65). The significantly fewer plastid nucleoids/generative cell in MS-5, compared to that of 7W and 301, correlates positively with the relatively poor male plastid transmission strength of this genotype. However, the difference between the mean number of plastid nucleoids/generative cell in 7W and 301 is not significant, yet 301 is a much stronger transmitter of male plastids than is 7W.
AB - A previous study on alfalfa determined that the number of plastids/generative cell does not necessarily correlate with male plastid transmission strength in a given genotype. The objectives of the present study were to learn (1) whether plastid nucleoid number/generative cell is comparable to the number of plastids/generative cell, and (2) whether plastid nucleoid number/generative cell correlates with known male plastid transmission behavior in three alfalfa genotypes. Our results, which were based upon 150 generative cells examined by DAPI/epifluorescence microscopy, indicate that the mean plastid nucleoid number/generative cell is much less than the mean number of plastids/generative cell in genotype 7W (60 nucleoids/264 plastids) and genotype 301 (54 nucleoids/165 plastids). In genotype MS-5, mean plastid nucleoid number/generative cell (45) is similar to the mean number of plastids/generative cell (65). The significantly fewer plastid nucleoids/generative cell in MS-5, compared to that of 7W and 301, correlates positively with the relatively poor male plastid transmission strength of this genotype. However, the difference between the mean number of plastid nucleoids/generative cell in 7W and 301 is not significant, yet 301 is a much stronger transmitter of male plastids than is 7W.
KW - Cytoplasmic inheritance
KW - DAPI fluorochrome
KW - Medicago sativa
KW - Plastid DNA
UR - http://www.scopus.com/inward/record.url?scp=0025803714&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025803714&partnerID=8YFLogxK
U2 - 10.1007/BF00309602
DO - 10.1007/BF00309602
M3 - Article
AN - SCOPUS:0025803714
VL - 19
SP - 399
EP - 401
JO - Current Genetics
JF - Current Genetics
SN - 0172-8083
IS - 5
ER -