Pathogen detection in the genomic era

Elizabeth M. Marlowe, Donna Wolk

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

In the 21st century, one of the greatest challenges to public health and clinical microbiologists is the rapid detection and identification of emerging and reemerging pathogens. Complex factors such as genetic variation in the host and pathogen, environmental changes, population pressures, and global travel can all influence the emergence of infectious diseases. The SARS epidemic of 2003 highlighted the potential of an emerging pathogen to spread globally in a very short time frame (Peruski and Peruski, 2003). The diagnostics of such infectious diseases has been greatly affected in the past 20 years. No longer is cultivation and microscopy the only means of detecting infectious agents. With the introduction of molecular diagnostics, the ability to detect minute amounts of microbial nucleic acids in clinical specimens has revolutionized clinical microbiology. In particular, the utility of PCR allows the detection and quantitation of specific agents in a matter of hours. PCR sequencing of specific segments of nucleic acid allows for the determination of specific drug resistance that now aids in guiding viral therapies.

Original languageEnglish (US)
Title of host publicationAdvanced Techniques in Diagnostic Microbiology
PublisherSpringer US
Pages505-523
Number of pages19
ISBN (Print)0387297413, 9780387297415
DOIs
StatePublished - 2006

Fingerprint

Nucleic Acids
Communicable Diseases
Polymerase Chain Reaction
Molecular Pathology
Population Dynamics
Microbiology
Drug Resistance
Microscopy
Public Health
Therapeutics

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Marlowe, E. M., & Wolk, D. (2006). Pathogen detection in the genomic era. In Advanced Techniques in Diagnostic Microbiology (pp. 505-523). Springer US. https://doi.org/10.1007/0-387-32892-0_28

Pathogen detection in the genomic era. / Marlowe, Elizabeth M.; Wolk, Donna.

Advanced Techniques in Diagnostic Microbiology. Springer US, 2006. p. 505-523.

Research output: Chapter in Book/Report/Conference proceedingChapter

Marlowe, EM & Wolk, D 2006, Pathogen detection in the genomic era. in Advanced Techniques in Diagnostic Microbiology. Springer US, pp. 505-523. https://doi.org/10.1007/0-387-32892-0_28
Marlowe EM, Wolk D. Pathogen detection in the genomic era. In Advanced Techniques in Diagnostic Microbiology. Springer US. 2006. p. 505-523 https://doi.org/10.1007/0-387-32892-0_28
Marlowe, Elizabeth M. ; Wolk, Donna. / Pathogen detection in the genomic era. Advanced Techniques in Diagnostic Microbiology. Springer US, 2006. pp. 505-523
@inbook{1375be10afdb444f8c71069544407560,
title = "Pathogen detection in the genomic era",
abstract = "In the 21st century, one of the greatest challenges to public health and clinical microbiologists is the rapid detection and identification of emerging and reemerging pathogens. Complex factors such as genetic variation in the host and pathogen, environmental changes, population pressures, and global travel can all influence the emergence of infectious diseases. The SARS epidemic of 2003 highlighted the potential of an emerging pathogen to spread globally in a very short time frame (Peruski and Peruski, 2003). The diagnostics of such infectious diseases has been greatly affected in the past 20 years. No longer is cultivation and microscopy the only means of detecting infectious agents. With the introduction of molecular diagnostics, the ability to detect minute amounts of microbial nucleic acids in clinical specimens has revolutionized clinical microbiology. In particular, the utility of PCR allows the detection and quantitation of specific agents in a matter of hours. PCR sequencing of specific segments of nucleic acid allows for the determination of specific drug resistance that now aids in guiding viral therapies.",
author = "Marlowe, {Elizabeth M.} and Donna Wolk",
year = "2006",
doi = "10.1007/0-387-32892-0_28",
language = "English (US)",
isbn = "0387297413",
pages = "505--523",
booktitle = "Advanced Techniques in Diagnostic Microbiology",
publisher = "Springer US",

}

TY - CHAP

T1 - Pathogen detection in the genomic era

AU - Marlowe, Elizabeth M.

AU - Wolk, Donna

PY - 2006

Y1 - 2006

N2 - In the 21st century, one of the greatest challenges to public health and clinical microbiologists is the rapid detection and identification of emerging and reemerging pathogens. Complex factors such as genetic variation in the host and pathogen, environmental changes, population pressures, and global travel can all influence the emergence of infectious diseases. The SARS epidemic of 2003 highlighted the potential of an emerging pathogen to spread globally in a very short time frame (Peruski and Peruski, 2003). The diagnostics of such infectious diseases has been greatly affected in the past 20 years. No longer is cultivation and microscopy the only means of detecting infectious agents. With the introduction of molecular diagnostics, the ability to detect minute amounts of microbial nucleic acids in clinical specimens has revolutionized clinical microbiology. In particular, the utility of PCR allows the detection and quantitation of specific agents in a matter of hours. PCR sequencing of specific segments of nucleic acid allows for the determination of specific drug resistance that now aids in guiding viral therapies.

AB - In the 21st century, one of the greatest challenges to public health and clinical microbiologists is the rapid detection and identification of emerging and reemerging pathogens. Complex factors such as genetic variation in the host and pathogen, environmental changes, population pressures, and global travel can all influence the emergence of infectious diseases. The SARS epidemic of 2003 highlighted the potential of an emerging pathogen to spread globally in a very short time frame (Peruski and Peruski, 2003). The diagnostics of such infectious diseases has been greatly affected in the past 20 years. No longer is cultivation and microscopy the only means of detecting infectious agents. With the introduction of molecular diagnostics, the ability to detect minute amounts of microbial nucleic acids in clinical specimens has revolutionized clinical microbiology. In particular, the utility of PCR allows the detection and quantitation of specific agents in a matter of hours. PCR sequencing of specific segments of nucleic acid allows for the determination of specific drug resistance that now aids in guiding viral therapies.

UR - http://www.scopus.com/inward/record.url?scp=77649225893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649225893&partnerID=8YFLogxK

U2 - 10.1007/0-387-32892-0_28

DO - 10.1007/0-387-32892-0_28

M3 - Chapter

AN - SCOPUS:77649225893

SN - 0387297413

SN - 9780387297415

SP - 505

EP - 523

BT - Advanced Techniques in Diagnostic Microbiology

PB - Springer US

ER -