Pattern duplications in larvae of the polyembryonic wasp Copidosoma floridanum

Miodrag Grbic, Lisa M Nagy, Michael Strand

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Copidosoma floridanum is a polyembryonic wasp that undergoes total cleavage of the egg followed by proliferation of blastomeres to produce up to 2,000 embryos from a single egg. This unusual mode of development raises several questions about how axial polarity is established in individual embryonic primordia. By examining embryonic development of larvae with duplicated structures (conjoined larvae), we determined that conjoined larvae form by mislocalization of two embryonic primordia to a common chamber of the extraembryonic membrane that surrounds individual embryos. Analysis of an anterior marker, Distalless, in mislocalized early embryos indicated that anterior structures form independently of one another. This suggests each embryonic primordium has some intrinsic polarity. However, during germband extension embryos usually fuse in register with each other, resulting in conjoined larvae with heads facing each other. Analysis of the posterior segmental marker, Engrailed, in conjoined embryos suggested that fusion in register initiates during germband extension. Thus, even though embryonic primordia initially have a random axial orientation, conjoined larvae usually possess a common orientation due to reorientation during germband extension. These observations suggest that differential cellular affinities during segmentation play an important role in embryo fusion.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalRoux's Archives of Developmental Biology
Volume206
Issue number4
StatePublished - 1996

Fingerprint

Copidosoma floridanum
Wasps
wasp
Larva
embryo
embryo (animal)
Embryonic Structures
larva
larvae
Ovum
egg
Extraembryonic Membranes
extraembryonic membranes
Blastomeres
blastomeres
embryonic development
segmentation
cleavage
Embryonic Development
embryogenesis

Keywords

  • Axis formation
  • Pattern formation
  • Polyembryony
  • Twinning

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Pattern duplications in larvae of the polyembryonic wasp Copidosoma floridanum. / Grbic, Miodrag; Nagy, Lisa M; Strand, Michael.

In: Roux's Archives of Developmental Biology, Vol. 206, No. 4, 1996, p. 281-287.

Research output: Contribution to journalArticle

@article{b837c275776741078b10a7fb38ebb81c,
title = "Pattern duplications in larvae of the polyembryonic wasp Copidosoma floridanum",
abstract = "Copidosoma floridanum is a polyembryonic wasp that undergoes total cleavage of the egg followed by proliferation of blastomeres to produce up to 2,000 embryos from a single egg. This unusual mode of development raises several questions about how axial polarity is established in individual embryonic primordia. By examining embryonic development of larvae with duplicated structures (conjoined larvae), we determined that conjoined larvae form by mislocalization of two embryonic primordia to a common chamber of the extraembryonic membrane that surrounds individual embryos. Analysis of an anterior marker, Distalless, in mislocalized early embryos indicated that anterior structures form independently of one another. This suggests each embryonic primordium has some intrinsic polarity. However, during germband extension embryos usually fuse in register with each other, resulting in conjoined larvae with heads facing each other. Analysis of the posterior segmental marker, Engrailed, in conjoined embryos suggested that fusion in register initiates during germband extension. Thus, even though embryonic primordia initially have a random axial orientation, conjoined larvae usually possess a common orientation due to reorientation during germband extension. These observations suggest that differential cellular affinities during segmentation play an important role in embryo fusion.",
keywords = "Axis formation, Pattern formation, Polyembryony, Twinning",
author = "Miodrag Grbic and Nagy, {Lisa M} and Michael Strand",
year = "1996",
language = "English (US)",
volume = "206",
pages = "281--287",
journal = "Development Genes and Evolution",
issn = "0949-944X",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Pattern duplications in larvae of the polyembryonic wasp Copidosoma floridanum

AU - Grbic, Miodrag

AU - Nagy, Lisa M

AU - Strand, Michael

PY - 1996

Y1 - 1996

N2 - Copidosoma floridanum is a polyembryonic wasp that undergoes total cleavage of the egg followed by proliferation of blastomeres to produce up to 2,000 embryos from a single egg. This unusual mode of development raises several questions about how axial polarity is established in individual embryonic primordia. By examining embryonic development of larvae with duplicated structures (conjoined larvae), we determined that conjoined larvae form by mislocalization of two embryonic primordia to a common chamber of the extraembryonic membrane that surrounds individual embryos. Analysis of an anterior marker, Distalless, in mislocalized early embryos indicated that anterior structures form independently of one another. This suggests each embryonic primordium has some intrinsic polarity. However, during germband extension embryos usually fuse in register with each other, resulting in conjoined larvae with heads facing each other. Analysis of the posterior segmental marker, Engrailed, in conjoined embryos suggested that fusion in register initiates during germband extension. Thus, even though embryonic primordia initially have a random axial orientation, conjoined larvae usually possess a common orientation due to reorientation during germband extension. These observations suggest that differential cellular affinities during segmentation play an important role in embryo fusion.

AB - Copidosoma floridanum is a polyembryonic wasp that undergoes total cleavage of the egg followed by proliferation of blastomeres to produce up to 2,000 embryos from a single egg. This unusual mode of development raises several questions about how axial polarity is established in individual embryonic primordia. By examining embryonic development of larvae with duplicated structures (conjoined larvae), we determined that conjoined larvae form by mislocalization of two embryonic primordia to a common chamber of the extraembryonic membrane that surrounds individual embryos. Analysis of an anterior marker, Distalless, in mislocalized early embryos indicated that anterior structures form independently of one another. This suggests each embryonic primordium has some intrinsic polarity. However, during germband extension embryos usually fuse in register with each other, resulting in conjoined larvae with heads facing each other. Analysis of the posterior segmental marker, Engrailed, in conjoined embryos suggested that fusion in register initiates during germband extension. Thus, even though embryonic primordia initially have a random axial orientation, conjoined larvae usually possess a common orientation due to reorientation during germband extension. These observations suggest that differential cellular affinities during segmentation play an important role in embryo fusion.

KW - Axis formation

KW - Pattern formation

KW - Polyembryony

KW - Twinning

UR - http://www.scopus.com/inward/record.url?scp=33747628570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747628570&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33747628570

VL - 206

SP - 281

EP - 287

JO - Development Genes and Evolution

JF - Development Genes and Evolution

SN - 0949-944X

IS - 4

ER -