Patterned protein films on poly(lipid) bilayers by microcontact printing

Eric E. Ross, James R. Joubert, Ronald J. Wysocki, Ken Nebesny, Tony Spatt, David F. O'Brien, Steven S Saavedra

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The use of polymerized lipid bilayers as substrates for microcontact printing (μCP) of protein films was investigated. We have previously shown that vesicle fusion of bis-SorbPC, a dienoate lipid, on glass and silica substrates, followed by redox-initiated radical polymerization, produces a planar supported lipid bilayer (PSLB) that is ultrastable [Ross, E. E.; Rozanski, L. J.; Spratt, T.; Liu, S.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 1752] and highly resistant to nonspecific adsorption of dissolved proteins [Ross, E. E.; Spratt, T.; Liu, S.; Rozanski, L. J.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 17661.] Here we demonstrate that μCP of bovine serum albumin (BSA) onto a dried poly(bis-SorbPC) PSLB from a poly(dimethylsiloxane) (PDMS) stamp produces a layer of strongly adsorbed protein, comparable in surface coverage to films printed on glass surfaces. Immobilization of proteins on poly(PSLB)s has potential applications in biosensing, and this work shows that direct μCP of proteins is a technically simple approach to create immobilized monolayers, as well as multilayers of different proteins.

Original languageEnglish (US)
Pages (from-to)1393-1398
Number of pages6
JournalBiomacromolecules
Volume7
Issue number5
DOIs
StatePublished - May 2006

Fingerprint

Printing
Lipid bilayers
Lipid Bilayers
Proteins
Glass
Substrates
Polydimethylsiloxane
Free radical polymerization
Bovine Serum Albumin
Polymerization
Silicon Dioxide
Immobilization
Lipids
Adsorption
Oxidation-Reduction
Monolayers
Multilayers
Fusion reactions
Silica

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Ross, E. E., Joubert, J. R., Wysocki, R. J., Nebesny, K., Spatt, T., O'Brien, D. F., & Saavedra, S. S. (2006). Patterned protein films on poly(lipid) bilayers by microcontact printing. Biomacromolecules, 7(5), 1393-1398. https://doi.org/10.1021/bm050727l

Patterned protein films on poly(lipid) bilayers by microcontact printing. / Ross, Eric E.; Joubert, James R.; Wysocki, Ronald J.; Nebesny, Ken; Spatt, Tony; O'Brien, David F.; Saavedra, Steven S.

In: Biomacromolecules, Vol. 7, No. 5, 05.2006, p. 1393-1398.

Research output: Contribution to journalArticle

Ross, EE, Joubert, JR, Wysocki, RJ, Nebesny, K, Spatt, T, O'Brien, DF & Saavedra, SS 2006, 'Patterned protein films on poly(lipid) bilayers by microcontact printing', Biomacromolecules, vol. 7, no. 5, pp. 1393-1398. https://doi.org/10.1021/bm050727l
Ross EE, Joubert JR, Wysocki RJ, Nebesny K, Spatt T, O'Brien DF et al. Patterned protein films on poly(lipid) bilayers by microcontact printing. Biomacromolecules. 2006 May;7(5):1393-1398. https://doi.org/10.1021/bm050727l
Ross, Eric E. ; Joubert, James R. ; Wysocki, Ronald J. ; Nebesny, Ken ; Spatt, Tony ; O'Brien, David F. ; Saavedra, Steven S. / Patterned protein films on poly(lipid) bilayers by microcontact printing. In: Biomacromolecules. 2006 ; Vol. 7, No. 5. pp. 1393-1398.
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