Pax2 expression occurs in renal medullary epithelial cells in vivo and in cell culture, is osmoregulated, and promotes osmotic tolerance

Qi Cai, Natalia I. Dmitrieva, Joan D. Ferraris, Heddwen L Brooks, Bas W M Van Balkom, Maurice Burg

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Pax2 is a transcription factor that is crucial for kidney development, and it is also expressed in the normal adult kidney, where its physiological function is unknown. In the present study, we find by cDNA microarray analysis that Pax2 expression in second-passage mouse inner-medullary epithelial cells is increased by a high NaCl concentration, which is significant because NaCl levels are normally high in the inner medulla in vivo, and varies with urinary concentration. Furthermore, a high NaCl concentration increases Pax2 mRNA and protein expression in mouse inner medullary collecting duct (mlMCD3) cells, and its transcriptional activity. Pax2 mRNA and protein expression is high in normal adult mouse renal inner medulla but much lower in renal cortex. Pax2 protein is present in collecting duct cells in both renal medulla and cortex and in thin descending limbs of Henle's loop in inner medulla. Treating Brattleboro rats with desamino-Cys-1,D-Arg-8 vasopressin, which increases inner-medullary NaCl concentration, causes a 4-fold increase in inner-medullary Pax2 protein. Treatment with furosemide, which decreases inner-medullary NaCl, reduces inner-medullary Pax2 mRNA and protein. Pax2-specific short interfering RNA increases high NaCl concentration-induced activation of caspase-3 and apoptotic bodies in mlMCD3 cells. We thus conclude that (i) Pax2 is expressed in normal renal medulla, (ii) its expression is regulated there by the normally high and variable NaCl concentration, and (iii) it protects renal medullary cells from high NaCl concentration-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)503-508
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number2
DOIs
StatePublished - Jan 11 2005

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Cell Culture Techniques
Epithelial Cells
Kidney
Proteins
Messenger RNA
Brattleboro Rats
Loop of Henle
Arginine Vasopressin
Furosemide
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Caspase 3
Small Interfering RNA
Transcription Factors
Apoptosis

Keywords

  • Apoptosis
  • Osmotic stress
  • Renal inner medulla

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Pax2 expression occurs in renal medullary epithelial cells in vivo and in cell culture, is osmoregulated, and promotes osmotic tolerance. / Cai, Qi; Dmitrieva, Natalia I.; Ferraris, Joan D.; Brooks, Heddwen L; Van Balkom, Bas W M; Burg, Maurice.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 2, 11.01.2005, p. 503-508.

Research output: Contribution to journalArticle

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