PBDX is the XG blood group gene

Nathan A. Ellis, Patricia Tippett, Alison Petty, Marion Reid, Polly A. Weller, Tian Z. Ye, James German, Peter N. Goodfellow, Stephen Thomas, George Banting

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalNature Genetics
Volume8
Issue number3
DOIs
StatePublished - Nov 1994

ASJC Scopus subject areas

  • Genetics

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    Ellis, N. A., Tippett, P., Petty, A., Reid, M., Weller, P. A., Ye, T. Z., German, J., Goodfellow, P. N., Thomas, S., & Banting, G. (1994). PBDX is the XG blood group gene. Nature Genetics, 8(3), 285-290. https://doi.org/10.1038/ng1194-285