PBDX is the XG blood group gene

Nathan Ellis, Patricia Tippett, Alison Petty, Marion Reid, Polly A. Weller, Tian Z. Ye, James German, Peter N. Goodfellow, Stephen Thomas, George Banting

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody- specific immobilization of antigen (ASIA) and immunoblot assays, the anti- peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalNature Genetics
Volume8
Issue number3
DOIs
StatePublished - Nov 1994
Externally publishedYes

Fingerprint

Blood Group Antigens
Peptides
Anti-Idiotypic Antibodies
Genes
Antigens
Hemagglutination
Membrane Proteins
Erythrocytes
Monoclonal Antibodies
Rabbits
Antibodies

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Ellis, N., Tippett, P., Petty, A., Reid, M., Weller, P. A., Ye, T. Z., ... Banting, G. (1994). PBDX is the XG blood group gene. Nature Genetics, 8(3), 285-290. https://doi.org/10.1038/ng1194-285

PBDX is the XG blood group gene. / Ellis, Nathan; Tippett, Patricia; Petty, Alison; Reid, Marion; Weller, Polly A.; Ye, Tian Z.; German, James; Goodfellow, Peter N.; Thomas, Stephen; Banting, George.

In: Nature Genetics, Vol. 8, No. 3, 11.1994, p. 285-290.

Research output: Contribution to journalArticle

Ellis, N, Tippett, P, Petty, A, Reid, M, Weller, PA, Ye, TZ, German, J, Goodfellow, PN, Thomas, S & Banting, G 1994, 'PBDX is the XG blood group gene', Nature Genetics, vol. 8, no. 3, pp. 285-290. https://doi.org/10.1038/ng1194-285
Ellis N, Tippett P, Petty A, Reid M, Weller PA, Ye TZ et al. PBDX is the XG blood group gene. Nature Genetics. 1994 Nov;8(3):285-290. https://doi.org/10.1038/ng1194-285
Ellis, Nathan ; Tippett, Patricia ; Petty, Alison ; Reid, Marion ; Weller, Polly A. ; Ye, Tian Z. ; German, James ; Goodfellow, Peter N. ; Thomas, Stephen ; Banting, George. / PBDX is the XG blood group gene. In: Nature Genetics. 1994 ; Vol. 8, No. 3. pp. 285-290.
@article{2e1043ebfb204471ad89fc4555380c70,
title = "PBDX is the XG blood group gene",
abstract = "We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody- specific immobilization of antigen (ASIA) and immunoblot assays, the anti- peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48{\%} homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.",
author = "Nathan Ellis and Patricia Tippett and Alison Petty and Marion Reid and Weller, {Polly A.} and Ye, {Tian Z.} and James German and Goodfellow, {Peter N.} and Stephen Thomas and George Banting",
year = "1994",
month = "11",
doi = "10.1038/ng1194-285",
language = "English (US)",
volume = "8",
pages = "285--290",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - PBDX is the XG blood group gene

AU - Ellis, Nathan

AU - Tippett, Patricia

AU - Petty, Alison

AU - Reid, Marion

AU - Weller, Polly A.

AU - Ye, Tian Z.

AU - German, James

AU - Goodfellow, Peter N.

AU - Thomas, Stephen

AU - Banting, George

PY - 1994/11

Y1 - 1994/11

N2 - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody- specific immobilization of antigen (ASIA) and immunoblot assays, the anti- peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

AB - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody- specific immobilization of antigen (ASIA) and immunoblot assays, the anti- peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

UR - http://www.scopus.com/inward/record.url?scp=0027996829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027996829&partnerID=8YFLogxK

U2 - 10.1038/ng1194-285

DO - 10.1038/ng1194-285

M3 - Article

VL - 8

SP - 285

EP - 290

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 3

ER -