PEAZ-1: A new human prostate neoplastic epithelial cell line

Monika Schmelz, Anne E. Cress, Jean Barrera, Kathy M. McDaniel, Tracy L. Davis, Laura Fuchs, Bruce L. Dalkin, Raymond B. Nagle

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

BACKGROUND. The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ-1 (prostate epithelial Arizona-1). METHODS. The PEAZ-1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies. RESULTS. PEAZ-1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ-1 cells express N-cadherin, α- and β-catenins, and p120 at cell-cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E-cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ-1 respond to ocadaic acid, a pro-apoptotic agent, by expression of p53. CONCLUSIONS. PEAZ-1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics.

Original languageEnglish (US)
Pages (from-to)79-92
Number of pages14
JournalProstate
Volume48
Issue number2
DOIs
StatePublished - Jul 1 2001

Keywords

  • Androgens
  • Cadherins
  • Catenins
  • In vitro cell culture
  • Integrins
  • Laminin 5
  • Prostate

ASJC Scopus subject areas

  • Oncology
  • Urology

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  • Cite this

    Schmelz, M., Cress, A. E., Barrera, J., McDaniel, K. M., Davis, T. L., Fuchs, L., Dalkin, B. L., & Nagle, R. B. (2001). PEAZ-1: A new human prostate neoplastic epithelial cell line. Prostate, 48(2), 79-92. https://doi.org/10.1002/pros.1084