PEAZ-1

A new human prostate neoplastic epithelial cell line

Monika Schmelz, Anne E Cress, Jean Barrera, Kathy M. McDaniel, Tracy L. Davis, Laura Fuchs, Bruce L. Dalkin, Raymond B Nagle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

BACKGROUND. The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ-1 (prostate epithelial Arizona-1). METHODS. The PEAZ-1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies. RESULTS. PEAZ-1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ-1 cells express N-cadherin, α- and β-catenins, and p120 at cell-cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E-cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ-1 respond to ocadaic acid, a pro-apoptotic agent, by expression of p53. CONCLUSIONS. PEAZ-1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics.

Original languageEnglish (US)
Pages (from-to)79-92
Number of pages14
JournalProstate
Volume48
Issue number2
DOIs
StatePublished - Jul 1 2001

Fingerprint

Prostate
Epithelial Cells
Cell Line
Cadherins
gamma Catenin
Keratin-8
Paxillin
Keratin-18
Vinculin
Carcinoma
Okadaic Acid
Desmosomes
Phosphotyrosine
Focal Adhesions
Vimentin
Prostatectomy
Diploidy
Androgens
Prostatic Neoplasms
Theoretical Models

Keywords

  • Androgens
  • Cadherins
  • Catenins
  • In vitro cell culture
  • Integrins
  • Laminin 5
  • Prostate

ASJC Scopus subject areas

  • Urology

Cite this

PEAZ-1 : A new human prostate neoplastic epithelial cell line. / Schmelz, Monika; Cress, Anne E; Barrera, Jean; McDaniel, Kathy M.; Davis, Tracy L.; Fuchs, Laura; Dalkin, Bruce L.; Nagle, Raymond B.

In: Prostate, Vol. 48, No. 2, 01.07.2001, p. 79-92.

Research output: Contribution to journalArticle

Schmelz, M, Cress, AE, Barrera, J, McDaniel, KM, Davis, TL, Fuchs, L, Dalkin, BL & Nagle, RB 2001, 'PEAZ-1: A new human prostate neoplastic epithelial cell line', Prostate, vol. 48, no. 2, pp. 79-92. https://doi.org/10.1002/pros.1084
Schmelz, Monika ; Cress, Anne E ; Barrera, Jean ; McDaniel, Kathy M. ; Davis, Tracy L. ; Fuchs, Laura ; Dalkin, Bruce L. ; Nagle, Raymond B. / PEAZ-1 : A new human prostate neoplastic epithelial cell line. In: Prostate. 2001 ; Vol. 48, No. 2. pp. 79-92.
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