PentaLyte® does not decrease heparinoid release but does decrease circulating thrombotic mediator activity associated with aortic occlusion-reperfusion in rabbits

Vance G Nielsen, Valerie E. Armstead, Brian T. Geary, Irina L. Opentanova

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hemorrhage and thrombosis are associated with major vascular and trauma surgery. Release of heparinoids and thrombotic mediators may contribute to these complications and have been described in rabbits after aortic occlusion-reperfusion. We hypothesized that the resuscitative fluid used could reduce heparinoid and thrombotic mediator release after aortic occlusion-reperfusion in rabbits as assessed by thromboelastographic variables (R, reaction time; α, angle; and G, a measure of clot strength). Anesthetized rabbits were administered lactated Ringer's solution (n = 8) or PentaLyte® (n = 8) at reperfusion after 30 min of ischemia. Blood was obtained before ischemia and after 30 min of reperfusion for thromboelastography under four conditions: 1) unmodified sample, 2) platelet inhibition, 3) heparinase, and 4) platelet inhibition and heparinase. During reperfusion, unmodified samples demonstrated a significant increase in R and decrease in α and G that was not affected by PentaLyte®. In the presence of heparinase, no significant fluid-specific thromboelastographic differences were noted. However, thrombotic mediator release (discerned by a decrease in R and an increase in α) during reperfusion in samples with platelet inhibition and heparinase was significantly attenuated by PentaLyte®. PentaLyte® administration does not decrease heparinoid release but does decrease thrombotic mediator release after aortic occlusion-reperfusion.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalAnesthesia and Analgesia
Volume92
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

Heparinoids
Reperfusion
Heparin Lyase
Rabbits
Blood Platelets
Ischemia
Thrombelastography
PentaLyte
Blood Vessels
Thrombosis
Hemorrhage
Wounds and Injuries

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

PentaLyte® does not decrease heparinoid release but does decrease circulating thrombotic mediator activity associated with aortic occlusion-reperfusion in rabbits. / Nielsen, Vance G; Armstead, Valerie E.; Geary, Brian T.; Opentanova, Irina L.

In: Anesthesia and Analgesia, Vol. 92, No. 2, 2001, p. 314-319.

Research output: Contribution to journalArticle

@article{27fb3b5da91d4f1a99ca1478123aae74,
title = "PentaLyte{\circledR} does not decrease heparinoid release but does decrease circulating thrombotic mediator activity associated with aortic occlusion-reperfusion in rabbits",
abstract = "Hemorrhage and thrombosis are associated with major vascular and trauma surgery. Release of heparinoids and thrombotic mediators may contribute to these complications and have been described in rabbits after aortic occlusion-reperfusion. We hypothesized that the resuscitative fluid used could reduce heparinoid and thrombotic mediator release after aortic occlusion-reperfusion in rabbits as assessed by thromboelastographic variables (R, reaction time; α, angle; and G, a measure of clot strength). Anesthetized rabbits were administered lactated Ringer's solution (n = 8) or PentaLyte{\circledR} (n = 8) at reperfusion after 30 min of ischemia. Blood was obtained before ischemia and after 30 min of reperfusion for thromboelastography under four conditions: 1) unmodified sample, 2) platelet inhibition, 3) heparinase, and 4) platelet inhibition and heparinase. During reperfusion, unmodified samples demonstrated a significant increase in R and decrease in α and G that was not affected by PentaLyte{\circledR}. In the presence of heparinase, no significant fluid-specific thromboelastographic differences were noted. However, thrombotic mediator release (discerned by a decrease in R and an increase in α) during reperfusion in samples with platelet inhibition and heparinase was significantly attenuated by PentaLyte{\circledR}. PentaLyte{\circledR} administration does not decrease heparinoid release but does decrease thrombotic mediator release after aortic occlusion-reperfusion.",
author = "Nielsen, {Vance G} and Armstead, {Valerie E.} and Geary, {Brian T.} and Opentanova, {Irina L.}",
year = "2001",
language = "English (US)",
volume = "92",
pages = "314--319",
journal = "Anesthesia and Analgesia",
issn = "0003-2999",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - PentaLyte® does not decrease heparinoid release but does decrease circulating thrombotic mediator activity associated with aortic occlusion-reperfusion in rabbits

AU - Nielsen, Vance G

AU - Armstead, Valerie E.

AU - Geary, Brian T.

AU - Opentanova, Irina L.

PY - 2001

Y1 - 2001

N2 - Hemorrhage and thrombosis are associated with major vascular and trauma surgery. Release of heparinoids and thrombotic mediators may contribute to these complications and have been described in rabbits after aortic occlusion-reperfusion. We hypothesized that the resuscitative fluid used could reduce heparinoid and thrombotic mediator release after aortic occlusion-reperfusion in rabbits as assessed by thromboelastographic variables (R, reaction time; α, angle; and G, a measure of clot strength). Anesthetized rabbits were administered lactated Ringer's solution (n = 8) or PentaLyte® (n = 8) at reperfusion after 30 min of ischemia. Blood was obtained before ischemia and after 30 min of reperfusion for thromboelastography under four conditions: 1) unmodified sample, 2) platelet inhibition, 3) heparinase, and 4) platelet inhibition and heparinase. During reperfusion, unmodified samples demonstrated a significant increase in R and decrease in α and G that was not affected by PentaLyte®. In the presence of heparinase, no significant fluid-specific thromboelastographic differences were noted. However, thrombotic mediator release (discerned by a decrease in R and an increase in α) during reperfusion in samples with platelet inhibition and heparinase was significantly attenuated by PentaLyte®. PentaLyte® administration does not decrease heparinoid release but does decrease thrombotic mediator release after aortic occlusion-reperfusion.

AB - Hemorrhage and thrombosis are associated with major vascular and trauma surgery. Release of heparinoids and thrombotic mediators may contribute to these complications and have been described in rabbits after aortic occlusion-reperfusion. We hypothesized that the resuscitative fluid used could reduce heparinoid and thrombotic mediator release after aortic occlusion-reperfusion in rabbits as assessed by thromboelastographic variables (R, reaction time; α, angle; and G, a measure of clot strength). Anesthetized rabbits were administered lactated Ringer's solution (n = 8) or PentaLyte® (n = 8) at reperfusion after 30 min of ischemia. Blood was obtained before ischemia and after 30 min of reperfusion for thromboelastography under four conditions: 1) unmodified sample, 2) platelet inhibition, 3) heparinase, and 4) platelet inhibition and heparinase. During reperfusion, unmodified samples demonstrated a significant increase in R and decrease in α and G that was not affected by PentaLyte®. In the presence of heparinase, no significant fluid-specific thromboelastographic differences were noted. However, thrombotic mediator release (discerned by a decrease in R and an increase in α) during reperfusion in samples with platelet inhibition and heparinase was significantly attenuated by PentaLyte®. PentaLyte® administration does not decrease heparinoid release but does decrease thrombotic mediator release after aortic occlusion-reperfusion.

UR - http://www.scopus.com/inward/record.url?scp=0034746476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034746476&partnerID=8YFLogxK

M3 - Article

C2 - 11159222

AN - SCOPUS:0034746476

VL - 92

SP - 314

EP - 319

JO - Anesthesia and Analgesia

JF - Anesthesia and Analgesia

SN - 0003-2999

IS - 2

ER -