Peptide detection of fungal functional amyloids in infected tissue

Melissa C. Garcia-Sherman, Nataliya Lysak, Alexandra Filonenko, Hazel Richards, Richard E Sobonya, Stephen A Klotz, Peter N. Lipke

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Many fungal cell adhesion proteins form functional amyloid patches on the surface of adhering cells. The Candida albicans Agglutinin-like sequence (Als) adhesins are exemplars for this phenomenon, and have amyloid forming sequences that are conserved between family members. The Als5p amyloid sequence mediates amyloid fibril formation and is critical for cell adhesion and biofilm formation, and is also present in the related adhesins Als1p and Als3p. We have developed a fluorescent peptide probe containing the conserved Als amyloid-forming sequence. This peptide bound specifically to yeast expressing Als5p, but not to cells lacking the adhesin. The probe bound to both yeast and hyphal forms of C. albicans. Dals1/Dals3 single and double deletion strains exhibited reduced fluorescence, indicating that probe binding required expression of these proteins. Additionally, the Als peptide specifically stained fungal cells in abscesses in autopsy sections. Counterstaining with calcofluor white showed colocalization with the amyloid peptide. In addition, fungi in autopsy sections derived from the gastrointestinal tract showed colocalization of the amyloid-specific dye thioflavin T and the fluorescent peptide. Collectively, our data demonstrate that we can exploit amyloid sequence specificity for detection of functional amyloids in situ.

Original languageEnglish (US)
JournalPLoS One
Volume9
Issue number1
DOIs
StatePublished - Jan 21 2014

Fingerprint

Fungal Proteins
amyloid
Amyloid
peptides
Tissue
adhesins
Agglutinins
agglutinins
Peptides
Cell adhesion
Candida albicans
cell adhesion
Cell Adhesion
Yeast
Autopsy
necropsy
Yeasts
yeasts
tissues
Conserved Sequence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Peptide detection of fungal functional amyloids in infected tissue. / Garcia-Sherman, Melissa C.; Lysak, Nataliya; Filonenko, Alexandra; Richards, Hazel; Sobonya, Richard E; Klotz, Stephen A; Lipke, Peter N.

In: PLoS One, Vol. 9, No. 1, 21.01.2014.

Research output: Contribution to journalArticle

Garcia-Sherman, MC, Lysak, N, Filonenko, A, Richards, H, Sobonya, RE, Klotz, SA & Lipke, PN 2014, 'Peptide detection of fungal functional amyloids in infected tissue', PLoS One, vol. 9, no. 1. https://doi.org/10.1371/journal.pone.0086067
Garcia-Sherman, Melissa C. ; Lysak, Nataliya ; Filonenko, Alexandra ; Richards, Hazel ; Sobonya, Richard E ; Klotz, Stephen A ; Lipke, Peter N. / Peptide detection of fungal functional amyloids in infected tissue. In: PLoS One. 2014 ; Vol. 9, No. 1.
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