Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens

Theresa M. Wizemann, Jackob Moskovitz, Barbara J. Pearce, Diana Cundell, Cindy G. Arvidson, Magdalene So, Herbert Weissbach, Nathan Brot, H. Robert Masure

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

Pathogenic bacteria rely on adhesins to bind to host tissues. Therefore, the maintenance of the functional properties of these extracellular macromolecules is essential for the pathogenicity of these microorganisms. We report that peptide methionine sulfoxide reductase (MsrA), a repair enzyme, contributes to the maintenance of adhesins in Streptococcus pneumoniae, Neisseria gonorrhoeae, and Escherichia coli. A screen of a library of pneumococcal mutants for loss of adherence uncovered a MsrA mutant with 75% reduced binding to GalNAcβ1-4Gal containing eukaryotic cell receptors that are present on type II lung cells and vascular endothelial cells. Subsequently, it was shown that an E. coli msrA mutant displayed decreased type I fimbriae-mediated, mannose-dependent, agglutination of erythrocytes. Previous work [Taha, M. K., Sn, M., Seifert, H. S., Billyard, E. and Marchal, C. (1988) EMBO J. 7, 4367-4378] has shown that mutants with defects in the pilA-pilB locus from N. gonorrhoeae were altered in their production of type IV pill. We show that pneumococcal MsrA and gonococcal PilB expressed in E. coli have MsrA activity. Together these data suggest that MsrA is required for the proper expression or maintenance of functional adhesins on the surfaces of these three major pathogenic bacteria.

Original languageEnglish (US)
Pages (from-to)7985-7990
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number15
DOIs
StatePublished - Jul 23 1996
Externally publishedYes

Keywords

  • Neisseria gonorrhoeae
  • Streptococcus pneumoniae
  • pilB
  • type 1 fimbriae
  • type IV pili

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens'. Together they form a unique fingerprint.

  • Cite this