Performance characteristics of beta-2-microglobulin measurements on a Beckman Immage analyzer with the DakoCytomation reagent kit

Barun K. De, Ernest Jimenez, Soumo De

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objective: Evaluation of serum beta-2-microglobulin (β2M) in an automated analyzer. Design and methods: The DakoCytomation β2M kit is an antibody based reagent intended for quantitative determination of β2M in serum and plasma by rate nephelometry. Results: The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope = 1.009, r = 0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L. Conclusion: The DakoCytomation reagent is acceptable to measure serum β2M.

Original languageEnglish (US)
Pages (from-to)1577-1581
Number of pages5
JournalClinical Biochemistry
Volume42
Issue number15
DOIs
StatePublished - Oct 1 2009

Keywords

  • Beta-2-microglobulin
  • DakoCytomation
  • Rate nephelometry
  • Tumor marker

ASJC Scopus subject areas

  • Clinical Biochemistry

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