Performance characteristics of the Dimension RxL iron and total iron-binding capacity methods

Barun K De, William L. Roberts

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Measurements of serum iron and total iron-binding capacity (TIBC) can be used to aid the diagnosis of iron deficiency and iron overload states. A variety of different methods for these measurements are commercially available. Methods: Linearity, imprecision and hemoglobin interference of homogeneous iron and TIBC methods on a Dimension® RxL analyzer were assessed. Method comparison studies were performed with a Vitros® 950 analyzer. Results: The Dimension RxL iron method was linear from 40 to 1000 μg/dl. The coefficient of variation of the iron and TIBC methods were <4% and <2%, respectively, at iron concentrations of 68 and 228 μg/dl and TIBC concentrations of 206 and 384 μg/dl. Comparison of the Vitros 950 and Dimension RxL iron methods gave a slope of 0.97, an intercept of 6.0 and r=0.99. Corresponding results for the TIBC methods were 1.02, -6.6 and r=0.97, respectively. Simulation of acute iron overload in vitro did not produce the same effect on TIBC measurements as in vivo overload. Iron recovery by the Dimension RxL method was reduced 40-60% by the addition of deferoxamine at a concentration of 200 μmol/l. Conclusions: The Dimension RxL assay provides acceptable measurements of iron and TIBC in routine patient samples. Iron measurements are unreliable in the presence of deferoxamine.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalClinica Chimica Acta
Volume333
Issue number1-2
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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Iron
Deferoxamine
Iron Overload
Hemoglobins
Assays

Keywords

  • Deferoxamine
  • Interference
  • Iron overload
  • Percent saturation
  • TIBC

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Performance characteristics of the Dimension RxL iron and total iron-binding capacity methods. / De, Barun K; Roberts, William L.

In: Clinica Chimica Acta, Vol. 333, No. 1-2, 01.07.2003, p. 51-57.

Research output: Contribution to journalArticle

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