Peripheral blood from human immunodeficiency virus type 1-infected patients displays diminished T cell generation capacity

Dawn R. Clark, Neil M. Ampel, Carrie A. Hallett, Venkat R K Yedavalli, Nafees - Ahmad, Dominick DeLuca

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An organ culture chimera system was used to assess the effect of human immunodeficiency virus type 1 (HIV-1) infection on the T cell-generation capacity of precursors derived from human peripheral blood. Peripheral blood mononuclear cells from HIV-1-infected patients and uninfected controls were placed on fetal thymus lobes of NOD/LtSz-scid/scid mice. Blood from the HIV- 1-infected patients consistently produced fewer CD4 and CD8 cells compared with blood from controls (P < .01). Addition of zidovudine to the cultures did not alter this profile. Limit dilution experiments suggested that there were fewer functional precursors in the infected patients. These results were not dependent on the patient's level of peripheral CD4 cells; even samples from patients with normal CD4 cell counts were unable to generate T cells in organ cultures. The results are consistent with a loss in the capacity of HIV-1-infected patients to produce functional T cell progenitors in their peripheral blood.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalJournal of Infectious Diseases
Volume176
Issue number3
StatePublished - 1997
Externally publishedYes

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HIV-1
T-Lymphocytes
Organ Culture Techniques
Zidovudine
Virus Diseases
CD4 Lymphocyte Count
Thymus Gland
Blood Cells

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Peripheral blood from human immunodeficiency virus type 1-infected patients displays diminished T cell generation capacity. / Clark, Dawn R.; Ampel, Neil M.; Hallett, Carrie A.; Yedavalli, Venkat R K; Ahmad, Nafees -; DeLuca, Dominick.

In: Journal of Infectious Diseases, Vol. 176, No. 3, 1997, p. 649-654.

Research output: Contribution to journalArticle

Clark, Dawn R. ; Ampel, Neil M. ; Hallett, Carrie A. ; Yedavalli, Venkat R K ; Ahmad, Nafees - ; DeLuca, Dominick. / Peripheral blood from human immunodeficiency virus type 1-infected patients displays diminished T cell generation capacity. In: Journal of Infectious Diseases. 1997 ; Vol. 176, No. 3. pp. 649-654.
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