Persistence of MS-2 and PRD-1 bacteriophages in an ultrapure water system

R. A. Governal, C. P. Gerba

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The persistence of bacteriophages MS-2 and PRD-1 was evaluated in tap water, in reverse osmosis (RO) permeate, and in three locations within an ultrapure water system; ultrapure samples included pre- and post-UV sterilization and post-mixed bed ion exchange tank. The inactivation rates for MS-2 were calculated as log10 reduction per hour and per day: k = - (log10 Ct/Co)/t. PRD-1 was found to persist with no significant loss of infectivity in all water purity environments evaluated. Inactivation of MS-2 was dependent on water quality and pH. Short-term inactivation rates for chlorinated tap water, post-RO, pre-UV, post-UV and post-ion exchange sample locations were 0.028, 0.455, 0.231, 0.191 and 0.168 log10 h-1, respectively. Long-term inactivation rates for chlorinated tap water, post-RO, pre-UV, post-UV and post-ion exchange sample locations were 0.485, 0.911, 0.605, 0.632 and 0.684 log10 day-1, respectively. Since phages were found to remain intact as well as to lyse in the ultrapure water environment, the phages have the potential to contaminate the ultrapure water environments of the microelectronics, pharmaceutical and power generation industries in both colloidal and dissolved form. Further work is proceeding to generate standardized and cost-effective methods to detect viruses in water environments.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalJournal of Industrial Microbiology and Biotechnology
Volume18
Issue number5
DOIs
StatePublished - Jan 1 1997

Keywords

  • Coliphage MS-2
  • Coliphage PRD-1
  • Inactivation
  • Ion exchange
  • Reverse osmosis
  • Ultraviolet light (UV)
  • Viruses

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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