Persufflation improves pancreas preservation when compared with the two-layer method

W. E. Scott, T. D. O'Brien, J. Ferrer-Fabrega, E. S. Avgoustiniatos, B. P. Weegman, T. Anazawa, S. Matsumoto, V. A. Kirchner, M. D. Rizzari, M. P. Murtaugh, T. M. Suszynski, T. Aasheim, L. S. Kidder, B. E. Hammer, S. G. Stone, L. A. Tempelman, D. E R Sutherland, B. J. Hering, Klearchos K Papas

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Islet transplantation is emerging as a promising treatment for patients with type 1 diabetes. It is important to maximize viable islet yield for each organ due to scarcity of suitable human donor pancreata, high cost, and the large dose of islets required for insulin independence. However, organ transport for 8 hours using the two-layer method (TLM) frequently results in low islet yields. Since efficient oxygenation of the core of larger organs (eg, pig, human) in TLM has recently come under question, we investigated oxygen persufflation as an alternative way to supply the pancreas with oxygen during preservation. Porcine pancreata were procured from donors after cardiac death and preserved by either TLM or persufflation for 24 hours and subsequently fixed. Biopsies collected from several regions of the pancreas were sectioned, stained with hematoxylin and eosin, and evaluated by a histologist. Persufflated tissues exhibited distended capillaries and significantly less autolysis/cell death relative to regions not exposed to persufflation or to tissues preserved with TLM. The histology presented here suggests that after 24 hours of preservation, persufflation dramatically improves tissue health when compared with TLM. These results indicate the potential for persufflation to improve viable islet yields and extend the duration of preservation, allowing more donor organs to be utilized.

Original languageEnglish (US)
Pages (from-to)2016-2019
Number of pages4
JournalTransplantation Proceedings
Volume42
Issue number6
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Pancreas
Tissue Donors
Swine
Oxygen
Autolysis
Islets of Langerhans Transplantation
Hematoxylin
Eosine Yellowish-(YS)
Type 1 Diabetes Mellitus
Histology
Cell Death
Insulin
Biopsy
Costs and Cost Analysis
Health
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Scott, W. E., O'Brien, T. D., Ferrer-Fabrega, J., Avgoustiniatos, E. S., Weegman, B. P., Anazawa, T., ... Papas, K. K. (2010). Persufflation improves pancreas preservation when compared with the two-layer method. Transplantation Proceedings, 42(6), 2016-2019. https://doi.org/10.1016/j.transproceed.2010.05.092

Persufflation improves pancreas preservation when compared with the two-layer method. / Scott, W. E.; O'Brien, T. D.; Ferrer-Fabrega, J.; Avgoustiniatos, E. S.; Weegman, B. P.; Anazawa, T.; Matsumoto, S.; Kirchner, V. A.; Rizzari, M. D.; Murtaugh, M. P.; Suszynski, T. M.; Aasheim, T.; Kidder, L. S.; Hammer, B. E.; Stone, S. G.; Tempelman, L. A.; Sutherland, D. E R; Hering, B. J.; Papas, Klearchos K.

In: Transplantation Proceedings, Vol. 42, No. 6, 07.2010, p. 2016-2019.

Research output: Contribution to journalArticle

Scott, WE, O'Brien, TD, Ferrer-Fabrega, J, Avgoustiniatos, ES, Weegman, BP, Anazawa, T, Matsumoto, S, Kirchner, VA, Rizzari, MD, Murtaugh, MP, Suszynski, TM, Aasheim, T, Kidder, LS, Hammer, BE, Stone, SG, Tempelman, LA, Sutherland, DER, Hering, BJ & Papas, KK 2010, 'Persufflation improves pancreas preservation when compared with the two-layer method', Transplantation Proceedings, vol. 42, no. 6, pp. 2016-2019. https://doi.org/10.1016/j.transproceed.2010.05.092
Scott WE, O'Brien TD, Ferrer-Fabrega J, Avgoustiniatos ES, Weegman BP, Anazawa T et al. Persufflation improves pancreas preservation when compared with the two-layer method. Transplantation Proceedings. 2010 Jul;42(6):2016-2019. https://doi.org/10.1016/j.transproceed.2010.05.092
Scott, W. E. ; O'Brien, T. D. ; Ferrer-Fabrega, J. ; Avgoustiniatos, E. S. ; Weegman, B. P. ; Anazawa, T. ; Matsumoto, S. ; Kirchner, V. A. ; Rizzari, M. D. ; Murtaugh, M. P. ; Suszynski, T. M. ; Aasheim, T. ; Kidder, L. S. ; Hammer, B. E. ; Stone, S. G. ; Tempelman, L. A. ; Sutherland, D. E R ; Hering, B. J. ; Papas, Klearchos K. / Persufflation improves pancreas preservation when compared with the two-layer method. In: Transplantation Proceedings. 2010 ; Vol. 42, No. 6. pp. 2016-2019.
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