Pet127 governs a 5′ →3′-exonuclease important in maturation of apocytochrome b mRNA in Saccharomyces cerevisiae

Zsuzsanna Fekete, Timothy P. Ellis, Melissa S. Schonauer, Carol L Dieckmann

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The details of mRNA maturation in Saccharomyces mitochondria are not well understood. All seven mRNAs are transcribed as part of multigenic units. The mRNAs are processed at a common 3′-dodecamer sequence, but the 5′-ends have seven different sequences. To investigate whether apocytochrome b (COB) mRNAis processed at the 5′-end from a longer precursor by an endonuclease or an exonuclease, a 64-nucleotide sequence, which is required for the protection of COB mRNA by the Cbp1 protein and is found at the 5′-end of the processed COB mRNA,was duplicated in tandem. The wild-type 64-nucleotide element functioned in either the upstream or downstream position when paired with a mutant element. In the tandem wild-type strain, the 5′-end of them RNA was at the 5′-end of the upstream unit, demonstrating that them RNA is processed by an exonuclease. Accumulation of precursor COB RNA in single and double element strains with a deletion of PET127 demonstrated that the encoded protein governs the 5′-exonuclease responsible for processing the precursor to the mature form.

Original languageEnglish (US)
Pages (from-to)3767-3772
Number of pages6
JournalJournal of Biological Chemistry
Volume283
Issue number7
DOIs
StatePublished - Feb 15 2008

Fingerprint

Exonucleases
Cytochromes b
Yeast
Saccharomyces cerevisiae
Messenger RNA
RNA
Nucleotides
Phosphodiesterase I
Saccharomyces
Mitochondria
Endonucleases
RNA Precursors
Proteins
Processing

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Pet127 governs a 5′ →3′-exonuclease important in maturation of apocytochrome b mRNA in Saccharomyces cerevisiae. / Fekete, Zsuzsanna; Ellis, Timothy P.; Schonauer, Melissa S.; Dieckmann, Carol L.

In: Journal of Biological Chemistry, Vol. 283, No. 7, 15.02.2008, p. 3767-3772.

Research output: Contribution to journalArticle

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AU - Dieckmann, Carol L

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