PGF stimulates FP prostanoid receptor mediated crosstalk between Ras/Raf signaling and Tcf transcriptional activation

Wei Xu, Chih Ling Chou, Davelene D. Israel, Anthony J. Hutchinson, John W Regan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Prostaglandin-F (PGF) is a product of the cyclooxygenase pathway and is a local signaling molecule that activates a G-protein coupled prostanoid receptor named FP. FP receptors can stimulate T-cell factor (Tcf) transcriptional activation by stabilization of β-catenin and can upregulate the expression of mRNA encoding cysteine-rich protein 61 (Cyr61), a secreted extracellular matrix protein that stimulates angiogenesis. We now show in both HEK cells and human microglial cells that the induction of Cyr61 protein expression by the human FP receptor utilizes a novel mechanism involving the activation of Ras and Raf followed by a MEK/ERK independent activation of Tcf signaling. The upregulation of Cyr61 in microglial cells may contribute to glioma tumorigenesis and could be a potential therapeutic target.

Original languageEnglish (US)
Pages (from-to)625-629
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume381
Issue number4
DOIs
StatePublished - Apr 17 2009

Fingerprint

Cysteine-Rich Protein 61
TCF Transcription Factors
Dinoprost
Crosstalk
Transcriptional Activation
Chemical activation
Up-Regulation
Catenins
Extracellular Matrix Proteins
Mitogen-Activated Protein Kinase Kinases
Prostaglandin-Endoperoxide Synthases
G-Protein-Coupled Receptors
GTP-Binding Proteins
Glioma
Prostaglandins
Carcinogenesis
Stabilization
Messenger RNA
Molecules
prostaglandin F2alpha receptor

Keywords

  • β-Catenin
  • Cyclooxygenase
  • Cysteine-rich protein 61
  • G-protein coupled receptors
  • Glioma
  • MAPK kinase
  • Microglial cells
  • Mitogen activated protein kinase
  • Prostaglandin
  • T-cell factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

PGF stimulates FP prostanoid receptor mediated crosstalk between Ras/Raf signaling and Tcf transcriptional activation. / Xu, Wei; Chou, Chih Ling; Israel, Davelene D.; Hutchinson, Anthony J.; Regan, John W.

In: Biochemical and Biophysical Research Communications, Vol. 381, No. 4, 17.04.2009, p. 625-629.

Research output: Contribution to journalArticle

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AU - Regan, John W

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