PH stepwise alkaline elution of DNA replication intermediates during S phase

Anne E. Cress, G. Tim Bowden

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have used a newly developed pH stepwise alkaline elution method to analyze the distribution of DNA replication intermediates during S phase. Using synchronized CHO (Chinese Hamster Ovary) cells, replicon-size replication intermediates (≤ 15S) reach a maximum in the first half of S phase. These intermediates are functional in that they are used to generate larger DNA fragments. The larger DNA fragments corresponding in size to replicating sections increase linearly with the progression of cells throughout S phase, corresponding to DNA replication kinetics previously obtained by CsCl density gradient analysis. These data will enable us to enrich for particular DNA replication fragments and identify the protein components associated with these intermediates.

Original languageEnglish (US)
Pages (from-to)845-853
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume102
Issue number3
DOIs
StatePublished - Oct 15 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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