Phage display technology in biosensor development

Scott C. Meyer, Indraneel Ghosh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The utility of phage display technology in biosensor development lies in the ability to rapidly discover novel peptide and protein modules that serve as molecular detection domains in sensor architectures. Phage display allows for connecting a protein or peptide (phenotype) to the DNA (genotype) that encodes it, allowing users to screen billion member libraries of compounds against targets of interest, while using the genetic information of the virus for both output identification and amplification. This process known as in vitro selection mimics natural selection through the use of selective pressure followed by the amplification of the selected mutant in a bacterial host cell. Because of the robust nature of phage, the output virions from a selection can sometimes be used directly in a biosensor. More commonly, the genetic information contained within the phage can be translated to a peptide, protein, or antibody that serves as the recognition domain in a biosensor. Several modalities of phage display have been utilized for discovering receptors for biosensors, including peptide display (low and high valency), antibody display, and small protein display. These phage display approaches have greatly aided in the development of novel biosensor receptors.

Original languageEnglish (US)
Title of host publicationRecognition Receptors in Biosensors
PublisherSpringer New York
Pages723-749
Number of pages27
ISBN (Print)9781441909183
DOIs
Publication statusPublished - 2010

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Keywords

  • Antibody
  • Avidin
  • In vitro selection
  • Landscape phage
  • M13 filamentous bacteriophage
  • Peptide library
  • Phage display
  • Protein grafting
  • Protein scaffold
  • Streptavidin
  • Zinc finger

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Meyer, S. C., & Ghosh, I. (2010). Phage display technology in biosensor development. In Recognition Receptors in Biosensors (pp. 723-749). Springer New York. https://doi.org/10.1007/978-1-4419-0919-0_18