Phase-transition thresholds and vaporization phenomena for ultrasound phase-change nanoemulsions assessed via high-speed optical microscopy

Paul S. Sheeran, Terry O Matsunaga, Paul A. Dayton

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Ultrasonically activated phase-change contrast agents (PCCAs) based on perfluorocarbon droplets have been proposed for a variety of therapeutic and diagnostic clinical applications. When generated at the nanoscale, droplets may be small enough to exit the vascular space and then be induced to vaporize with high spatial and temporal specificity by externally-applied ultrasound. The use of acoustical techniques for optimizing ultrasound parameters for given applications can be a significant challenge for nanoscale PCCAs due to the contributions of larger outlier droplets. Similarly, optical techniques can be a challenge due to the sub-micron size of nanodroplet agents and resolution limits of optical microscopy. In this study, an optical method for determining activation thresholds of nanoscale emulsions based on the in vitro distribution of bubbles resulting from vaporization of PCCAs after single, short (<10 cycles) ultrasound pulses is evaluated. Through ultra-high-speed microscopy it is shown that the bubbles produced early in the pulse from vaporized droplets are strongly affected by subsequent cycles of the vaporization pulse, and these effects increase with pulse length. Results show that decafluorobutane nanoemulsions with peak diameters on the order of 200 nm can be optimally vaporized with short pulses using pressures amenable to clinical diagnostic ultrasound machines.

Original languageEnglish (US)
Pages (from-to)4513-4534
Number of pages22
JournalPhysics in Medicine and Biology
Volume58
Issue number13
DOIs
StatePublished - Jul 7 2013

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Volatilization
Phase Transition
Contrast Media
Microscopy
Fluorocarbons
Emulsions
Blood Vessels
Ultrasonography
Blood Pressure
Therapeutics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Medicine(all)

Cite this

Phase-transition thresholds and vaporization phenomena for ultrasound phase-change nanoemulsions assessed via high-speed optical microscopy. / Sheeran, Paul S.; Matsunaga, Terry O; Dayton, Paul A.

In: Physics in Medicine and Biology, Vol. 58, No. 13, 07.07.2013, p. 4513-4534.

Research output: Contribution to journalArticle

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