Phenotypic and functional analysis of 1,25-dihydroxyvitamin d3 receptor mediated modulation of the human myeloma cell line rpmi 8226

Jean Francois Rossi, Brain G.M. Durie, Christophe Duperray, Theodore Braich, Samuel L. Marion, J. Wesley Pike, Mark R. Haussler, Charles Janbon, Regis Bataille

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Abstract

Several recent studies have demonstrated the presence of specific receptors for the 1,25-dihydroxyvitamin D3 (calcitriol) in activated normal lymphocytes. By DNA cellulose chromatography, we show evidence of such specific receptors in the human myeloma cell line RPMI 8226. Nanomolar concentrations of 1,25-dihydroxyvitamin D3 reduce the proliferation of RPMI 8226 cells significantly and simultaneously induce the appearance of both new properties and phenotype expression, such as butyrate esterase, enhanced expression of CD20 (Bl), CD15 (Leu-Ml) antigens and X chains, and decreased expression of the PCI antigen using microfluorometric analysis. But such an increased expression of membrane X chains was not associated with an enhanced secretion of X chains. Furthermore, the bone resorbing activity produced normally by RPMI 8226 cells was reduced significantly after 1,25-dihydroxyvitamin D3 treatment. The possible mechanisms and significance of these new functional and phenotypic properties are discussed with respect to the B-cell lineage.

Original languageEnglish (US)
Pages (from-to)1213-1216
Number of pages4
JournalCancer Research
Volume48
Issue number5
StatePublished - Mar 1988

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Rossi, J. F., Durie, B. G. M., Duperray, C., Braich, T., Marion, S. L., Wesley Pike, J., Haussler, M. R., Janbon, C., & Bataille, R. (1988). Phenotypic and functional analysis of 1,25-dihydroxyvitamin d3 receptor mediated modulation of the human myeloma cell line rpmi 8226. Cancer Research, 48(5), 1213-1216.