Phenotypic variation and host interactions of Xenorhabdus bovienii SS-2004, the entomopathogenic symbiont of Steinernema jollieti nematodes

Darby R. Sugar, Kristen E. Murfin, John M. Chaston, Aaron W. Andersen, Gregory R. Richards, Limaris deLéon, James A. Baum, William P. Clinton, Steven Forst, Barry S. Goldman, Karina C. Krasomil-Osterfeld, Steven Slater, S. Patricia Stock, Heidi Goodrich-Blair

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Xenorhabdus bovienii (SS-2004) bacteria reside in the intestine of the infective-juvenile (IJ) stage of the entomopathogenic nematode, Steinernema jollieti. The recent sequencing of the X.bovienii genome facilitates its use as a model to understand host - symbiont interactions. To provide a biological foundation for such studies, we characterized X.bovienii in vitro and host interaction phenotypes. Within the nematode host X.bovienii was contained within a membrane bound envelope that also enclosed the nematode-derived intravesicular structure. Steinernema jollieti nematodes cultivated on mixed lawns of X.bovienii expressing green or DsRed fluorescent proteins were predominantly colonized by one or the other strain, suggesting the colonizing population is founded by a few cells. Xenorhabdus bovienii exhibits phenotypic variation between orange-pigmented primary form and cream-pigmented secondary form. Each form can colonize IJ nematodes when cultured in vitro on agar. However, IJs did not develop or emerge from Galleria mellonella insects infected with secondary form. Unlike primary-form infected insects that were soft and flexible, secondary-form infected insects retained a rigid exoskeleton structure. Xenorhabdus bovienii primary and secondary form isolates are virulent towards Manduca sexta and several other insects. However, primary form stocks present attenuated virulence, suggesting that X.bovienii, like Xenorhabdus nematophila may undergo virulence modulation.

Original languageEnglish (US)
Pages (from-to)924-939
Number of pages16
JournalEnvironmental Microbiology
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2012

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Sugar, D. R., Murfin, K. E., Chaston, J. M., Andersen, A. W., Richards, G. R., deLéon, L., Baum, J. A., Clinton, W. P., Forst, S., Goldman, B. S., Krasomil-Osterfeld, K. C., Slater, S., Stock, S. P., & Goodrich-Blair, H. (2012). Phenotypic variation and host interactions of Xenorhabdus bovienii SS-2004, the entomopathogenic symbiont of Steinernema jollieti nematodes. Environmental Microbiology, 14(4), 924-939. https://doi.org/10.1111/j.1462-2920.2011.02663.x