Pheromone-evoked potentials and oscillations in the antennal lobes of the sphinx moth Manduca sexta

T. Heinbockel, P. Kloppenburg, John G Hildebrand

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Using intra- and extracellular recording methods, we studied the activity of pheromone-responsive projection neurons in the antennal lobe of the moth Manduca sexta. Intracellularly recorded responses of neurons to antennal stimulation with the pheromone blend characteristically included both inhibitory and excitatory stages of various strengths. To observe the activity of larger groups of neurons, we recorded responses extracellularly in the macroglomerular complex of the antennal lobe. The macroglomerular complex is part of a specialized olfactory subsystem and the site of first-order central processing of sex-pheromonal information. Odors such as the pheromone blend and host-plant (tobacco) volatiles gave rise to evoked potentials that were reproducible upon repeated antennal stimulation. Evoked potentials showed overriding high-frequency oscillations when the antenna was stimulated with the pheromone blend or with either one of the two key pheromone components. The frequency of the oscillations was in the range of 30-50 Hz. Amplitude and frequency of the oscillations varied during the response to pheromonal stimulation. Recording intracellular and extracellular activity simultaneously revealed phase-locking of action potentials to potential oscillations. The results suggest that the activity of neurons of the macroglomerular complex was temporally synchronized, potentially to strengthen the pheromone signal and to improve olfactory perception.

Original languageEnglish (US)
Pages (from-to)703-714
Number of pages12
JournalJournal of Comparative Physiology A
Volume182
Issue number6
DOIs
StatePublished - Jun 1998

Fingerprint

antennal lobe
Manduca
Sphingidae
evoked potentials
Moths
Pheromones
Manduca sexta
pheromone
pheromone blends
Evoked Potentials
moth
pheromones
oscillation
neurons
Neurons
Olfactory Perception
action potentials
antennae
moths
tobacco

Keywords

  • Evoked potential
  • Glomerulus
  • Olfaction
  • Oscillation
  • Pheromone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Physiology
  • Physiology (medical)
  • Behavioral Neuroscience
  • Neuroscience(all)

Cite this

Pheromone-evoked potentials and oscillations in the antennal lobes of the sphinx moth Manduca sexta. / Heinbockel, T.; Kloppenburg, P.; Hildebrand, John G.

In: Journal of Comparative Physiology A, Vol. 182, No. 6, 06.1998, p. 703-714.

Research output: Contribution to journalArticle

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