Phloroglucinol-based colorimetry of xylose in plasma and urine compared with a specific gas-chromatographic procedure

S. L. Johnson, M. Bliss, Michael Mayersohn, K. A. Conrad

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A commonly used method for xylose has several disadvantages, including use of thiourea which is a carcinogen. We examined an alternative colorimetric method in which phloroglucinol (1,3,5-trihydroxybenzene) is the chromogenic reagent and compared it with a specific gas-chromatographic procedure for use with blood and urine samples from subjects receiving xylose either orally or intravenously. Results by these methods correlated well, both for plasma (r2 = 0.97) and urine (r2 = 0.99). At concentrations >50 mg/L for plasma (slope = 1.005) and >350 mg/L for urine (slope = 0.99) the slopes were not significantly different from 1 or the intercepts different from zero. We recommend this method as a safer and more expedient alternative to the method currently used.

Original languageEnglish (US)
Pages (from-to)1571-1574
Number of pages4
JournalClinical Chemistry
Volume30
Issue number9
StatePublished - 1984

Fingerprint

Phloroglucinol
Colorimetry
Xylose
Gases
Urine
Chromogenics
Plasmas
Thiourea
Carcinogens
Blood

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Phloroglucinol-based colorimetry of xylose in plasma and urine compared with a specific gas-chromatographic procedure. / Johnson, S. L.; Bliss, M.; Mayersohn, Michael; Conrad, K. A.

In: Clinical Chemistry, Vol. 30, No. 9, 1984, p. 1571-1574.

Research output: Contribution to journalArticle

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