Abstract
A commonly used method for xylose has several disadvantages, including use of thiourea which is a carcinogen. We examined an alternative colorimetric method in which phloroglucinol (1,3,5-trihydroxybenzene) is the chromogenic reagent and compared it with a specific gas-chromatographic procedure for use with blood and urine samples from subjects receiving xylose either orally or intravenously. Results by these methods correlated well, both for plasma (r2 = 0.97) and urine (r2 = 0.99). At concentrations >50 mg/L for plasma (slope = 1.005) and >350 mg/L for urine (slope = 0.99) the slopes were not significantly different from 1 or the intercepts different from zero. We recommend this method as a safer and more expedient alternative to the method currently used.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1571-1574 |
| Number of pages | 4 |
| Journal | Clinical Chemistry |
| Volume | 30 |
| Issue number | 9 |
| State | Published - 1984 |
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ASJC Scopus subject areas
- Clinical Biochemistry
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Phloroglucinol-based colorimetry of xylose in plasma and urine compared with a specific gas-chromatographic procedure. / Johnson, S. L.; Bliss, M.; Mayersohn, Michael; Conrad, K. A.
In: Clinical Chemistry, Vol. 30, No. 9, 1984, p. 1571-1574.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phloroglucinol-based colorimetry of xylose in plasma and urine compared with a specific gas-chromatographic procedure
AU - Johnson, S. L.
AU - Bliss, M.
AU - Mayersohn, Michael
AU - Conrad, K. A.
PY - 1984
Y1 - 1984
N2 - A commonly used method for xylose has several disadvantages, including use of thiourea which is a carcinogen. We examined an alternative colorimetric method in which phloroglucinol (1,3,5-trihydroxybenzene) is the chromogenic reagent and compared it with a specific gas-chromatographic procedure for use with blood and urine samples from subjects receiving xylose either orally or intravenously. Results by these methods correlated well, both for plasma (r2 = 0.97) and urine (r2 = 0.99). At concentrations >50 mg/L for plasma (slope = 1.005) and >350 mg/L for urine (slope = 0.99) the slopes were not significantly different from 1 or the intercepts different from zero. We recommend this method as a safer and more expedient alternative to the method currently used.
AB - A commonly used method for xylose has several disadvantages, including use of thiourea which is a carcinogen. We examined an alternative colorimetric method in which phloroglucinol (1,3,5-trihydroxybenzene) is the chromogenic reagent and compared it with a specific gas-chromatographic procedure for use with blood and urine samples from subjects receiving xylose either orally or intravenously. Results by these methods correlated well, both for plasma (r2 = 0.97) and urine (r2 = 0.99). At concentrations >50 mg/L for plasma (slope = 1.005) and >350 mg/L for urine (slope = 0.99) the slopes were not significantly different from 1 or the intercepts different from zero. We recommend this method as a safer and more expedient alternative to the method currently used.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021718840&partnerID=8YFLogxK
M3 - Article
C2 - 6467574
AN - SCOPUS:0021718840
VL - 30
SP - 1571
EP - 1574
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 9
ER -