Phosphate transport by plasma membranes of enterocytes during development: Role of 1,25-dihydroxycholecalciferol

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19 Scopus citations

Abstract

The present studies were designed to investigate phosphate transport across the brush border and basolateral membranes of enterocytes and to determine the effect of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] on these processes in suckling and adolescent rats. Vitamin D deficiency was induced in suckling rats by feeding pregnant dams a vitamin D-deficient diet 48 h after insemination; they were then kept in the dark. Vitamin D deficiency in the adolescent rats was induced by feeding the vitamin D- deficient diet to weanling rats for 4 wk. Vmax values for Na+-dependent phosphate uptake in the brush border membranes of vitamin D-deficient and 1,25(OH)2D3-injected suckling rats was 0.7 ± 0.1 and 1.5 ± 0.2 nmol · mg protein-1 · 10 s-1 (P < 0.01), respectively; Vmax values in adolescent rats were 0.2 ± 0.05 and 0.36 ± 0.04 nmol · mg protein-1 · 10 s-1 (P < 0.05), respectively. Vmax values for Na+-dependent phosphate uptake in basolateral membranes of vitamin D-deficient and 1,25(OH)2D3-treated suckling rats were 0.006 ± 0.001 and 0.047 ± 0.006 nmol · mg protein-1 · 10 s-1 (P < 0.01).

Original languageEnglish (US)
Pages (from-to)873-877
Number of pages5
JournalAmerican Journal of Clinical Nutrition
Volume55
Issue number4
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • Phosphate
  • enterocyte
  • vitamin D

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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