Phospholipid bilayer membranes: Deuterium and carbon-13 NMR spectroscopy

Avigdor Leftin, Xiaolin Xu, Michael F Brown

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Solid-state 13C and 2H NMR measurements allow characterization of lipid membrane structure and dynamics and offer unique insights into their biophysical properties. Structural and dynamical formalisms provide a model-free reduction of 2H and 13C NMR lineshape and relaxation measurements in terms of mean-square amplitudes (order parameters) and reduced spectral densities (correlation times). Segmental order parameters are obtained from residual quadrupolar couplings (RQCs) and residual dipolar couplings (RDCs) that provide direct information about bilayer structural properties. Application of a mean-torque model gives the area per lipid and volumetric hydrocarbon thickness, and is important for validating molecular dynamics (MD) simulations. Moreover, NMR relaxation rates yield complementary information about multiscale bilayer dynamics if treated in a unified manner. Combination of frequency-dependent spin-lattice relaxation rates and anisotropic spin couplings allow unified interpretations of complementary 2H and 13C NMR experiments. Relaxometric measurements show that lipid mobility and collective order fluctuations are implicitly governed by the viscoelastic nature of the liquid-crystalline ensemble. Notably, the bilayer interior has a microviscosity similar to liquid hydrocarbons. Collective bilayer excitations emerge over mesoscopic length scales between the molecular and bilayer dimensions and are important for lipid organization and protein interactions.

Original languageEnglish (US)
Pages (from-to)199-214
Number of pages16
JournaleMagRes
Volume3
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Deuterium
Phospholipids
Lipids
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Carbon
Nuclear magnetic resonance
Hydrocarbons
Membranes
Torque
Molecular Dynamics Simulation
Membrane Lipids
Membrane structures
Spin-lattice relaxation
Spectral density
Liquids
Molecular dynamics
Structural properties
Crystalline materials
Proteins

Keywords

  • magnetic dipolar coupling
  • molecular dynamics
  • nuclear spin-lattice relaxation
  • order parameter
  • phospholipid bilayer
  • quadrupolar coupling
  • solid-state NMR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Biomedical Engineering
  • Biochemistry
  • Radiology Nuclear Medicine and imaging

Cite this

Phospholipid bilayer membranes : Deuterium and carbon-13 NMR spectroscopy. / Leftin, Avigdor; Xu, Xiaolin; Brown, Michael F.

In: eMagRes, Vol. 3, No. 3, 2014, p. 199-214.

Research output: Contribution to journalArticle

Leftin, Avigdor ; Xu, Xiaolin ; Brown, Michael F. / Phospholipid bilayer membranes : Deuterium and carbon-13 NMR spectroscopy. In: eMagRes. 2014 ; Vol. 3, No. 3. pp. 199-214.
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