Phospholipid Biosynthesis in Growing and Non-growing Conditions in Candida albicans

Fariba Mirbod, Shigeru Nakashima, Shunji Mori, Yasuo Kitajima, Yoshinori Nozawa

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The major membrane phospholipids of Candida albicans (C. albicans) are phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (Pi), and phosphatidylserine (PS). In Saccharomyces cerevisiae (S. cerevisiae), the most abundant component PC is considered to be synthesized mainly by two pathways; stepwise methylation pathway of PE produced from PS by decarboxylation (de novo pathway) and diacylglycerol (DG)/CDP-choline pathway. However, relatively little information is available regarding the phospholipid biosynthesis in C. albicans. In order to clarify the phospholipid metabolism of this organism, we have investigated the 32 Pi incorporation into different phospholipids by continuous and pulse-labeling in cells grown in the synthetic medium (SM) and yeast extractproteose peptone-dextrose (YPD) medium. The continuous labeling with 32Pi in YPD medium showed that the decreases in radioactivity in acidic phospholipids (PA, PI and PS) were followed by the great increase in PC, suggesting the existence of de novo pathway. In SM cultured cells showing no growth, PS and PI were heavily labeled, while PC was much less labeled. By the switch from SM to YPD, the rapid increase in 32P-labeled PC occurred at the expense of PI and PS, suggesting that the principal PC synthesis for membrane biogenesis is via the de novo pathway in C. albicans.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalNippon Ishinkin Gakkai Zasshi
Volume36
Issue number1
DOIs
StatePublished - 1995

Keywords

  • Candida albicans
  • Phospholipids
  • Synthetic medium (SM)

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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