Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads

Jean Vion-Dury, Jean Philippe Galons, Jacques Fantini, Sylviane Confort-Gouny, Mostafa Kriat, Patrick J. Cozzone

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalBrain Research
Volume493
Issue number1
DOIs
StatePublished - Jul 24 1989
Externally publishedYes

Keywords

  • Cell culture
  • Glioma cell line
  • Microcarrier
  • Nuclear magnetic resonance spectrocopy
  • P-
  • Phosphorylated compound

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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