Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation

Eva V. Varga, Dagmar Stropova, Marc Rubenzik, Sue Waite, William R Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

An immunoprecipitation method was used to measure [32P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human δ-opioid receptor. Chronic SNC 80 ((+)-4-[(αR)-α-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethyl-benzamide) (1 μM, 24 h) treatment increased the incorporation of [32P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 μM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
JournalEuropean Journal of Pharmacology
Volume364
Issue number2-3
DOIs
StatePublished - Jan 8 1999
Externally publishedYes

Fingerprint

naltrindole
Opioid Receptors
Immunoprecipitation
Phosphorylation
Blocking Antibodies
Cricetulus
Electrophoresis
Ovary
Proteins
Gels
Phosphates
Peptides
adenylyl cyclase 6
4-(alpha-(4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl)-N,N-diethylbenzamide
benzamide

Keywords

  • Adenylyl cyclase VI
  • Opioid receptor
  • Phosphorylation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation. / Varga, Eva V.; Stropova, Dagmar; Rubenzik, Marc; Waite, Sue; Roeske, William R; Yamamura, Henry I.

In: European Journal of Pharmacology, Vol. 364, No. 2-3, 08.01.1999.

Research output: Contribution to journalArticle

Varga, Eva V. ; Stropova, Dagmar ; Rubenzik, Marc ; Waite, Sue ; Roeske, William R ; Yamamura, Henry I. / Phosphorylation of adenylyl cyclase VI upon chronic δ-opioid receptor stimulation. In: European Journal of Pharmacology. 1999 ; Vol. 364, No. 2-3.
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