Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

Jiaqi Shi, John W B Hershey, Mark A Nelson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

Original languageEnglish (US)
Pages (from-to)971-977
Number of pages7
JournalFEBS Letters
Volume583
Issue number6
DOIs
StatePublished - Mar 18 2009

Fingerprint

Eukaryotic Initiation Factors
Phosphorylation
Cyclin-Dependent Kinases
Eukaryotic Initiation Factor-3
Apoptosis
Protein Kinases
Assays
Association reactions

Keywords

  • Apoptosis
  • Cyclin-dependent kinase 11
  • eIF3f
  • Melanoma
  • Phosphorylation
  • Translation initiation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis. / Shi, Jiaqi; Hershey, John W B; Nelson, Mark A.

In: FEBS Letters, Vol. 583, No. 6, 18.03.2009, p. 971-977.

Research output: Contribution to journalArticle

@article{d307a7eb52c04d1ca9173b48bc59aad5,
title = "Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis",
abstract = "eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).",
keywords = "Apoptosis, Cyclin-dependent kinase 11, eIF3f, Melanoma, Phosphorylation, Translation initiation",
author = "Jiaqi Shi and Hershey, {John W B} and Nelson, {Mark A}",
year = "2009",
month = "3",
day = "18",
doi = "10.1016/j.febslet.2009.02.028",
language = "English (US)",
volume = "583",
pages = "971--977",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis

AU - Shi, Jiaqi

AU - Hershey, John W B

AU - Nelson, Mark A

PY - 2009/3/18

Y1 - 2009/3/18

N2 - eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

AB - eIF3f is a subunit of eukaryotic initiation factor 3 (eIF3). We previously showed that eIF3f is phosphorylated by cyclin dependent kinase 11 (CDK11p46) which is an important effector in apoptosis. Here, we identified a second eIF3f phosphorylation site (Thr119) by CDK11p46 during apoptosis. We demonstrated that eIF3f is directly phosphorylated by CDK11p46 in vivo. Phosphorylation of eIF3f plays an important role in regulating its function in translation and apoptosis. Phosphorylation of eIF3f enhances the association of eIF3f with the core eIF3 subunits during apoptosis. Our data suggested that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Structured summary: MINT-6948874: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948891: EIF3b (uniprotkb:P55884) physically interacts (MI:0218) with EIF3c (uniprotkb:Q99613), EIF3a (uniprotkb:Q14152) and EIF3f (uniprotkb:O00303) by anti bait coimmunoprecipitation (MI:0006). MINT-6948836, MINT-6948849, MINT-6948862: CDK11p46 (uniprotkb:P21127) phosphorylates (MI:0217) EIF3f (uniprotkb:O00303) by protein kinase assay (MI:0424).

KW - Apoptosis

KW - Cyclin-dependent kinase 11

KW - eIF3f

KW - Melanoma

KW - Phosphorylation

KW - Translation initiation

UR - http://www.scopus.com/inward/record.url?scp=61749091373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61749091373&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2009.02.028

DO - 10.1016/j.febslet.2009.02.028

M3 - Article

C2 - 19245811

AN - SCOPUS:61749091373

VL - 583

SP - 971

EP - 977

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 6

ER -